Plant Cell Reports

, Volume 20, Issue 1, pp 79–84

Investigation on the induction of 14-3-3 in white spruce

  • G. Lapointe
  • M. D. Luckevich
  • A. Séguin
GENETICS AND GENOMICS

DOI: 10.1007/s002990000275

Cite this article as:
Lapointe, G., Luckevich, M. & Séguin, A. Plant Cell Reports (2001) 20: 79. doi:10.1007/s002990000275

Abstract

 A cDNA encoding a 14-3-3 protein was isolated from white spruce. The corresponding polypeptide contains several motifs that are conserved in this type of protein and is predicted to be 260 amino acids in length. Multiple banding in Southern blot analysis suggests that the gene encoding this cDNA is, in fact, part of a small family of genes. Wounding and chitosan treatment of spruce plants followed by Northern blot analysis indicates that these stimuli caused the accumulation of 14-3-3 mRNA. In addition, cell suspension cultures treated with methyl jasmonate showed up-regulation of 14-3-3-encoding mRNA. Chitosan and methyl jasmonate are both signalling molecules in the activation of plant defense response genes. Therefore, our results suggest a possible role for this 14-3-3 protein in the pathogen defense response of coniferous trees.

Keywords Coniferous trees Picea glauca 14-3-3 proteins Pathogen defense response 

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • G. Lapointe
    • 1
  • M. D. Luckevich
    • 1
  • A. Séguin
    • 1
  1. 1.Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 rue du P.E.P.S., P.O. Box 3800, Sainte-Foy, Quebec, Canada G1V 4C7 e-mail: seguin@cfl.forestry.ca Fax: +1-418-6485849CA

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