Plant Cell Reports

, Volume 19, Issue 12, pp 1155–1159 | Cite as

Doubled haploid plant production from unpollinated ovules of sugar beet (Beta vulgaris L.)

  • S. Gürel
  • E. Gürel
  • Z. Kaya
CELL BIOLOGY AND MORPHOGENESIS

Abstract

 The effects of cold pretreatment, AgNO3 and activated charcoal on haploid plant production from unpollinated sugar beet ovules were investigated. Both cold pretreatment and the addition of charcoal increased the frequency of embryo formation, whereas AgNO3 decreased or completely inhibited it. Colchicine (50, 100, 150 or 500 mg l–1) and trifluralin (1.7, 3.4 or 5.0 mg l–1) for 12, 24, 36 or 48 h were compared in agar-solidified, agarose-solidified or liquid media. Although colchicine gave a higher doubling rate (25.3%) than trifluralin (18.2%), the difference was not significant. Both agents were more effective when used in liquid (29.1%) than agarose-solidified medium (20.7%) and agar-solidified medium (15.4%). A treatment duration of 48 h was significantly more effective (27.5%) than 12 h (13.6%) but it was not different from 24 h (16.3%) or 36 h (18.6%).

KeywordsBeta vulgaris L. Ovule culture Haploid plant production Chromosome doubling 
AbbreviationsAPM: Amiprophosmethyl BAP: 6-Benzylaminopurine MS: Murashige and Skoog (1962) NAA: α-Naphthaleneacetic acid 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • S. Gürel
    • 1
  • E. Gürel
    • 2
  • Z. Kaya
    • 3
  1. 1.Sugar Institute, Department of Plant Breeding, 06790 Etimesgut, Ankara, TurkeyTR
  2. 2.Abant Izzet Baysal University, Department of Biology, 14280 Bolu, TurkeyTR
  3. 3.Middle East Technical University, Department of Biology, 06110 Ankara, Turkey e-mail: kayaz@metu.edu.tr Fax: +90-312-2101289TR

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