Plant Cell Reports

, Volume 19, Issue 8, pp 815–820 | Cite as

Agrobacterium-mediated transformation of monocot and dicot plants using the NCR promoter derived from soybean chlorotic mottle virus

  • H. Fukuoka
  • T. Ogawa
  • I. Mitsuhara
  • T. Iwai
  • K. Isuzugawa
  • Yoko Nishizawa
  • Y. Gotoh
  • Yaeko Nishizawa
  • A. Tagiri
  • M. Ugaki
  • M. Ohshima
  • H. Yano
  • N. Murai
  • Y. Niwa
  • T. Hibi
  • Y. Ohashi
GENETICS AND GENOMICS

Abstract

The NCR promoter (PNCR) from soybean chlorotic mottle virus (SoyCMV) was used to express the selectable marker, neomycin phosphotransferase (nptII) gene, in Agrobacterium-mediated transformation of both monocot (rice) and dicot (tobacco) plants. A multi-cloning site for insertion of a gene of interest into the binary vector pTN is located proximal to the right border region of T-DNA. When chimeric genes under the control of other strong promoters were located in a head-to-head orientation to the PNCR-nptII gene, kanamycin-resistant tobacco shoots were generated more efficiently than when using the original pTN vectors. This suggests that the enhancer-like sequences in the promoters adjacent to PNCR may promote expression of the PNCR-nptII gene.

Key words Soybean chlorotic mottle virus Agrobacterium-mediated transformation NCR promoter Transgenic rice Transgenic tobacco 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • H. Fukuoka
    • 1
  • T. Ogawa
    • 2
  • I. Mitsuhara
    • 2
  • T. Iwai
    • 2
  • K. Isuzugawa
    • 3
  • Yoko Nishizawa
    • 2
  • Y. Gotoh
    • 2
  • Yaeko Nishizawa
    • 2
  • A. Tagiri
    • 2
  • M. Ugaki
    • 2
  • M. Ohshima
    • 4
  • H. Yano
    • 1
  • N. Murai
    • 5
  • Y. Niwa
    • 6
  • T. Hibi
    • 7
  • Y. Ohashi
    • 2
  1. 1.Chugoku National Agricultural Experiment Station, 6-12-1 Nishifukatsu, Fukuyama, Hiroshima 721-8514, JapanJP
  2. 2.National Institute of Agrobiological Resources, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, JapanJP
  3. 3.Yamagata Prefectural Horticultural Experiment Station, Shima-minami, Shima, Sagae, Yamagata 991-0043, JapanJP
  4. 4.Hokuriku National Agricultural Experiment Station, 1-2-1 Inada, Johetsu, Niigata 943-0193, JapanJP
  5. 5.Department of Plant Pathology and Crop Physiology, 302 Life Sciences Building, Louisiana State University and LSU Agricultural Center, Baton Rouge, LA 70803-1720, USAUS
  6. 6.Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 51-1 Yada, Shizuoka, Shizuoka 422-8526, JapanJP
  7. 7.Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanJP

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