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Time-dependent behavior of phenylpropanoid pathway in response to methyl jasmonate in Scrophularia striata cell cultures

  • Ehsan Sadeghnezhad
  • Mohsen SharifiEmail author
  • Hassan Zare-Maivan
  • Najmeh Ahmadian Chashmi
Original Article

Abstract

Key message

MeJA triggers a time-dependent behavior of the phenylpropanoid compounds.

Abstract

Plant cells produce a large number of metabolites in response to environmental factors. The cellular responses to environmental changes are orchestrated by signaling molecules, such as methyl jasmonate (MeJA). To understand how the MeJA changes the behavior of amino acids, carbohydrates, and phenylpropanoid compounds such as phenolic acids, phenylethanoid-glycosides, and flavonoids in Scrophularia striata cells; we monitored the metabolic responses for different times of exposure. In this study, we performed a time course analysis of metabolites and enzymes in S. striata cells exposed to MeJA (100 µM) and evaluated the metabolic flux towards carbon-rich secondary metabolites production. Moreover, we calculated the biosynthetic energy cost for free amino acids. Our results indicated that MeJA accelerates the sucrose degradation and directs the metabolic fluxes towards a pool of flavonoids and phenylethanoid glycosides through a change in enzyme behavior in the entry point and center of the phenylpropanoid pathway. MeJA also decreased and then raised the amino acid biosynthesis cost in S. striata cells in a time-dependent manner, indicating the cells evolve to utilize amino acids more economically by reducing cell growth. Finally, we classified the marked changes in the metabolites level and enzyme activities into three groups including early-, late-, and oscillatory-response groups to MeJA and summarized our findings as a model depicting pathway interactions during MeJA elicitation. Determination of metabolic levels in response to MeJA suggests that the changes in metabolic responses are time-dependent.

Keywords

Energy cost Metabolic response Methyl jasmonate Phenylpropanoid Scrophularia striata 

Abbreviations

C-INV

Cytoplasmic or alkaline invertase

4CL

4-Coumarate: CoA ligase

FC

Functional clusters

MeJA

Methyl jasmonate

PAL

Phenylalanine ammonia-lyase

PCA

Principal component analysis

PheGs

Phenylethanoid glycosides

PLS-DA

Partial least squares discriminate analysis

ROS

Reactive oxygen species

TAL

Tyrosine ammonia lyase

V-INV

Vacuolar or acidic invertase

Notes

Acknowledgements

This work was supported by Tarbiat Modares University.

Author contribution statement

ES, MS, and HZM designed the research and collected the experimental data. ES and NAC performed the biological and computational analysis. ES wrote the original manuscript and all co-authors edited the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

299_2019_2486_MOESM1_ESM.docx (4.8 mb)
Supplementary material 1 (DOCX 4910 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ehsan Sadeghnezhad
    • 1
  • Mohsen Sharifi
    • 1
    Email author
  • Hassan Zare-Maivan
    • 1
  • Najmeh Ahmadian Chashmi
    • 2
  1. 1.Department of Plant Biology, Faculty of Biological SciencesTarbiat Modares UniversityTehranIran
  2. 2.Interfaculty Institute of BiochemistryTübingenGermany

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