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Plant Cell Reports

, Volume 38, Issue 9, pp 1165–1180 | Cite as

Overexpression of AtAGT1 promoted root growth and development during seedling establishment

  • Rui Wang
  • Lin Yang
  • Xiaofang Han
  • Yuhong Zhao
  • Ling Zhao
  • Beibei Xiang
  • Yerong Zhu
  • Yanling Bai
  • Yong WangEmail author
Original Article

Abstract

Key message

Arabidopsis photorespiratory gene AtAGT1 is important for the growth and development of root, the non-photosynthetic organ, and it is involved in a complex metabolic network and salt resistance.

Abstract

AtAGT1 in Arabidopsis encodes an aminotransferase that has a wide range of donor:acceptor combinations, including Asn:glyoxylate. Although it is one of the photorespiratory genes, its encoding protein has been suggested to function also in roots to metabolize Asn. However, experimental data are still lacking. In this study, we investigated experimentally the function of AtAGT1 in roots and our results uncovered its importance in root development during seedling establishment after seed germination. Overexpression of AtAGT1 in roots promoted both the growth of primary root and outgrowth of lateral roots. To further elucidate the molecular mechanisms underlying, amino acid content and gene expression in roots were analyzed, and results revealed that AtAGT1 is involved in a complex metabolic network and salt resistance of roots.

Keywords

AtAGT1 Seedling establishment Asn Ser Salt tolerance ABA signaling 

Notes

Acknowledgements

We thank Prof. Hermann Bauwe and Dr. Stefan Timm, Rostock University, for giving us valuable suggestions in preparing this article and providing us transgenic Arabidopsis lines with leaf-specific expression of FpSGT. We also wish to thank Prof. Ningning Wang, Nankai University, for providing us the Arabidopsis PRP3 promoter. This work was supported by National Natural Science Foundation of China (Grant no. 31270296), PPP Project of CSC-DAAD (2013) and 111 Project B08011.

Author contribution statement

YW designed the experiments; RW, LY, LZ, YZ and XH performed experiments; BX, YZ and YB contributed to technical support, discussion of the experiments and manuscript; YW, RW and XH wrote this MS.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

299_2019_2435_MOESM1_ESM.docx (982 kb)
Supplementary material 1 (DOCX 982 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Rui Wang
    • 1
  • Lin Yang
    • 2
  • Xiaofang Han
    • 1
  • Yuhong Zhao
    • 1
  • Ling Zhao
    • 1
  • Beibei Xiang
    • 3
  • Yerong Zhu
    • 1
  • Yanling Bai
    • 1
  • Yong Wang
    • 1
    Email author
  1. 1.College of Life SciencesNankai UniversityTianjinChina
  2. 2.Tianjin Key Laboratory of Animal and Plant ResistanceTianjin Normal UniversityTianjinChina
  3. 3.School of Chinese MateriaTianjin University of Traditional Chinese MedicineTianjinChina

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