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Plant Cell Reports

, Volume 38, Issue 9, pp 1109–1125 | Cite as

Over-expression of the Brachypodium ASR gene, BdASR4, enhances drought tolerance in Brachypodium distachyon

  • Jin Seok Yoon
  • Jae Yoon Kim
  • Man Bo Lee
  • Yong Weon SeoEmail author
Original Article

Abstract

Key message

BdASR4 expression was up-regulated during abiotic stress and hormone treatments. Plants over-expressing BdASR4 improved drought tolerant. BdASR4 may regulate antioxidant activities and transcript levels of stress-related and abscisic acid-responsive genes.

Abstract

Abiotic stress conditions negatively affect plant growth and developmental processes, causing a reduction in crop productivity. The abscisic acid-, stress-, ripening-induced (ASR) proteins play important roles in the protection of plants from abiotic stress. Brachypodium distachyon L. is a well-studied monocot model plant. However, ASR proteins of Brachypodium have not been widely studied. In this study, five ASR genes of Brachypodium plant were cloned and characterized. The BdASR genes were expressed in response to various abiotic stresses and hormones. In particular, BdASR4 was shown to encode a protein containing a nuclear localization signal in its C-terminal region, which enabled protein localization in the nucleus. To further examine functions of BdASR4, transgenic Brachypodium plants harboring BdASR4 were generated. Over-expression of BdASR4 was associated with strong drought tolerance, and plants over-expressing BdASR4 preserved more water and displayed higher antioxidant enzyme activities than did the wild-type plants. The transcript levels of stress-responsive genes, reactive oxygen species scavenger-associated genes, and abscisic acid-responsive genes tended to be higher in transgenic plants than in WT plants. Moreover, plants over-expressing BdASR4 were hypersensitive to exogenous abscisic acid at the germination stage. Taken together, these findings suggest multiple roles for BdASR4 in the plant response to drought stress by regulating antioxidant enzymes and the transcription of stress- and abscisic acid-responsive genes.

Keywords

Drought Brachypodium distachyon L. Abscisic acid Enzymatic antioxidants Stress-responsive genes 

Abbreviations

ABA

Abscisic acid

ASR

Aba-, stress-, ripening-induced

APX

Ascorbate peroxidase

GFP

Green fluorescent protein

MDA

Malondialdehyde

PEG

Polyethylene glycol

RWC

Relative water content

SOD

Superoxide dismutase

Notes

Acknowledgements

This work was supported by the Rural Development Administration, Republic of Korea through their project “Cooperative Research Program for Agriculture Science & Technology Development (Project no. PJ013244)”.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

299_2019_2429_MOESM1_ESM.docx (2.4 mb)
Supplementary material 1 (DOCX 2478 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Jin Seok Yoon
    • 1
  • Jae Yoon Kim
    • 1
    • 2
  • Man Bo Lee
    • 1
  • Yong Weon Seo
    • 1
    Email author
  1. 1.Department of Biosystems and BiotechnologyKorea UniversitySeoulRepublic of Korea
  2. 2.Department of Plant ResourcesKongju National UniversityYesanRepublic of Korea

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