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Overexpression of an ABA-dependent grapevine bZIP transcription factor, VvABF2, enhances osmotic stress in Arabidopsis

  • Jinyi Liu
  • Jinjin Chu
  • Chuangju Ma
  • Yueting Jiang
  • Yuanchun Ma
  • Jinsong Xiong
  • Zong-Ming ChengEmail author
Original Article

Abstract

Key message

Overexpression of grapevine VvABF2 gene could enhance osmotic stress tolerance in Arabidopsis thaliana but fully required for ABA signaling.

Abstract

The abscisic acid (ABA)-dependent AREB/ABF-SnRK2 pathway has been demonstrated to play a pivotal role in response to osmotic stress in model plants. However, its function in other specific species, for example grapevine, has not been fully characterized. In this study, grapevine (Vitis vinifera L.) ABSCISIC ACID RESPONSE ELEMENT-BINDING FACTOR2 (VvABF2), a homologous gene of AREB/ABFs form Arabidopsis, was isolated and constitutively expressed in Arabidopsis under the control of the cauliflower mosaic virus 35S promoter. The VvABF2 transgenic Arabidopsis plants showed to be more sensitive to exogenous ABA compared to wild type plants and exhibited significant osmotic tolerance, like polyethylene glycol (PEG) and drought but fully required ABA for signaling. This fact was further confirmed by its downstream gene expression assays. In addition, the determination of ROS antioxidant enzymes (including SOD, POD and CAT) and the MDA of transgenic lines indicated that overexpression of VvABF2 in Arabidopsis significantly increased ROS scavenging ability and thereby reduced the cell membrane damage, which might be ABA-independent. Our results provide evidence that VvABF2 has a similar function to the Arabidopsis homolog in response to osmotic stresses, and that there is a similar ancestral function of this gene in ABA-dependent response to stresses in grapevine.

Keywords

Grapevine Arabidopsis thaliana VvABF2 Osmotic stress ABA 

Notes

Acknowledgements

This study was supported by the National Natural Science Foundation of China (31501737, 31601739) and in part by Priority Academic Program Development of Modern Horticulture Science in Jiangsu Province, China (CX(14)2051) and the China Postdoctoral Science Foundation (2016M600425).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Supplementary material 1 (TIF 898 KB)
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Supplementary material 4 (XLSX 9 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Jinyi Liu
    • 1
  • Jinjin Chu
    • 1
  • Chuangju Ma
    • 1
  • Yueting Jiang
    • 1
  • Yuanchun Ma
    • 1
  • Jinsong Xiong
    • 1
  • Zong-Ming Cheng
    • 1
    • 2
    Email author
  1. 1.College of HorticultureNanjing Agricultural UniversityNanjingChina
  2. 2.Department of Plant SciencesUniversity of TennesseeKnoxvilleUSA

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