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Abnormal tapetum development and energy metabolism associated with sterility in SaNa-1A CMS of Brassica napus L.

  • Kun Du
  • Yuyue Xiao
  • Qier Liu
  • Xinyue Wu
  • Jinjin Jiang
  • Jian Wu
  • Yujie Fang
  • Yang Xiang
  • Youping WangEmail author
Original Article

Abstract

Key message

Abnormal tapetum degradation and anther development in cytoplasmic male sterility SaNa-1A are the main reasons for the anther abortion.

Abstract

SaNa-1A is a novel cytoplasmic male sterility (CMS) line of Brassica napus derived from somatic hybrids of B. napus-Sinapis alba, and SaNa-1B is the corresponding maintainer line. Ultrastructural comparison between developing anthers of sterile and maintainer lines revealed abnormal subcellular structure of pollen mother cells (PMCs) in the CMS line. The PMC volume and size of nucleus and nucleolus in the CMS line were smaller than those in the maintainer line. The abnormal tapetum cell development and delayed tapetum degradation inhibited microspore development. Finally, anther abortion in the CMS line occurred. Physiological and biochemical analyses of developing anthers and mitochondria revealed that over-accumulation of reactive oxygen species (ROS) in the SaNa-1A and deficiency in antioxidant enzyme system aggravated the oxidization of membrane lipids, resulting in malondialdehyde (MDA) accumulation in anthers. High MDA content in the CMS line was toxic to the cells. ROS accumulation in SaNa-1A also affected anther development. Abnormal structure and function of terminal oxidase, which participates in the electron transport chain of mitochondrial membrane, were observed and affected the activity of cytochrome c oxidase and F1F0-ATPase, which inhibited ATP biosynthesis. Proline deficiency in SaNa-1A also affected anther development. Few hybridization signals of programmed cell death (PCD) in tetrads of SaNa-1A were identified using TdT-mediated dUTP Nick-End Labeling assay. PCD was not obvious in tapetum cells of SaNa-1A until the unicellular stage. These results validated the cytological differences mentioned above, and proved that abnormal tapetum degradation and anther development in SaNa-1A were the main reasons for the anther abortion.

Keywords

Brassica napus Cytoplasmic male sterility Tapetum Programmed cell death Energy metabolism 

Notes

Acknowledgements

This research was funded by the National Natural Science Foundation of China (31330057, 31571699, 31771824), the National Key Research and Development Program of China (2016YFD0102000, 2016YFD0101000), the National Key Basic Research Program of China (2015CB150201), the Natural Science Foundation of Jiangsu Province (BE2018356, BK20180101), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Kun Du
    • 1
  • Yuyue Xiao
    • 1
  • Qier Liu
    • 1
  • Xinyue Wu
    • 1
  • Jinjin Jiang
    • 1
  • Jian Wu
    • 1
  • Yujie Fang
    • 1
  • Yang Xiang
    • 2
  • Youping Wang
    • 1
    Email author
  1. 1.Jiangsu Provincial Key Laboratory of Crop Genetics and PhysiologyYangzhou UniversityYangzhouChina
  2. 2.Guizhou Rapeseed InstituteGuizhou Academy of Agricultural SciencesGuiyangChina

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