Agrobacterium-mediated transient transformation of Bienertia sinuspersici to assay recombinant protein distribution between dimorphic chloroplasts

  • Diana Wimmer
  • Philipp Bohnhorst
  • Daniela Impe
  • Inhwan Hwang
  • Sascha OffermannEmail author
Focus Article


Key message

Bienertia sinuspersici, a species capable of performing C4 photosynthesis within individual photosynthetic cells, can be transformed with Agrobacterium allowing for the analysis of subcellular protein distribution under preservation of the unique cellular morphology.



Work in the S.O. lab was supported by the Deutsche Forschungsgemeinschaft (OF 106/1-1) and S.O. and I.H were funded by the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. 0109532016)”, Rural Development Administration, Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

299_2019_2375_MOESM1_ESM.docx (6.7 mb)
Supplementary material 1 (DOCX 6827 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute for BotanyLeibniz University HannoverHannoverGermany
  2. 2.Genebank Department, Cryo and Stress Biology GroupLeibniz Institute of Plant Genetics and Crop Plant Research (IPK-Gatersleben)Stadt SeelandGermany
  3. 3.Division of Integrative Biosciences and BiotechnologyPohang University of Science and TechnologyPohangSouth Korea

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