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Plant Cell Reports

, Volume 38, Issue 2, pp 147–159 | Cite as

Expression of seven carbonic anhydrases in red alga Gracilariopsis chorda and their subcellular localization in a heterologous system, Arabidopsis thaliana

  • Md. Abdur Razzak
  • JunMo Lee
  • Dong Wook Lee
  • Jeong Hee Kim
  • Hwan Su Yoon
  • Inhwan HwangEmail author
Original Article

Abstract

Key message

Red alga, Gracilariopsis chorda, contains seven carbonic anhydrases that can be grouped into α-, β- and γ-classes.

Abstract

Carbonic anhydrases (CAHs) are metalloenzymes that catalyze the reversible hydration of CO2. These enzymes are present in all living organisms and play roles in various cellular processes, including photosynthesis. In this study, we identified seven CAH genes (GcCAHs) from the genome sequence of the red alga Gracilariopsis chorda and characterized them at the molecular, cellular and biochemical levels. Based on sequence analysis, these seven isoforms were categorized into four α-class, one β-class, and two γ-class isoforms. RNA sequencing revealed that of the seven CAHs isoforms, six genes were expressed in G. chorda in light at room temperature. In silico analysis revealed that these seven isoforms localized to multiple subcellular locations such as the ER, mitochondria and cytosol. When expressed as green fluorescent protein fusions in protoplasts of Arabidopsis thaliana leaf cells, these seven isoforms showed multiple localization patterns. The four α-class GcCAHs with an N-terminal hydrophobic leader sequence localized to the ER and two of them were further targeted to the vacuole. GcCAHβ1 with no noticeable signal sequence localized to the cytosol. The two γ-class GcCAHs also localized to the cytosol, despite the presence of a predicted presequence. Based on these results, we propose that the red alga G. chorda also employs multiple CAH isoforms for various cellular processes such as photosynthesis.

Keywords

Carbonic anhydrase Subcellular localization CO2 Phylogenetic tree Gracilariopsis chorda Red algae 

Notes

Acknowledgements

This work was supported by grants from the National Research Foundation of Korea, Ministry of Science and ICT (No. 2016R1E1A1A02922014), and from the Collaborative Genome Program of the Korea Institute of Marine Science and Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (MOF) (20180430). Dong Wook Lee was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant number: PJ01335801), Rural Development Administration, Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Division of Integrative Biosciences and BiotechnologyPohang University of Science and TechnologyPohangSouth Korea
  2. 2.Department of Biological SciencesSungkyunkwan UniversitySuwonSouth Korea
  3. 3.Department of Biochemistry and Molecular Biology, College of DentistryKyung Hee UniversitySeoulSouth Korea
  4. 4.Department of Life and Nanopharmaceutical Sciences, Graduate SchoolKyung Hee UniversitySeoulSouth Korea
  5. 5.Department of Life SciencesPohang University of Science and TechnologyPohangSouth Korea

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