Plant Cell Reports

, Volume 38, Issue 1, pp 1–13 | Cite as

Comparative transcriptome analysis shows the defense response networks regulated by miR482b

  • Ning Jiang
  • Jun Cui
  • Guanglei Yang
  • Xiaoli He
  • Jun Meng
  • Yushi LuanEmail author
Original Article


Key message

The transcriptomic profile in the leaves of miR482b-overexpressing tomato plants revealed that miR482b may suppress alpha-linolenic acid metabolism, cysteine and methionine metabolism, plant–pathogen interaction, and the MAPK pathway to reduce resistance to Phytophthora infestans.


Our previous study showed that tomato miR482b acted as a negative regulator during tomato resistance to Phytophthora infestans by silencing NBS-LRR genes. To investigate pathways related to miR482b, the transcriptomic profile of tomato plants that overexpressed miR482b was constructed. A total of 47,124,670 raw sequence reads from the leaves of miR482b-overexpressing tomato plants were generated by Illumina sequencing. A total of 746 genes in miR482b-overexpressing tomato plants were found to show significantly differential expression relative to those in wild-type tomato plants, including 132 up-regulated genes and 614 down-regulated genes. GO and KEGG enrichment analyses showed that plant–pathogen interaction, the MAPK pathway, and the pathways related to JA and ET biosynthesis were affected by miR482b in tomato. qRT-PCR results showed that all the enriched genes in these pathways were down-regulated in tomato plants that overexpressed miR482b and up-regulated in tomato plants that overexpressed an NBS-LRR gene (Soly02g036270.2, the target gene of miR482b). After P. infestans infection, the expression of the enriched genes showed a time-dependent response, and the genes played different roles between resistant tomato (Solanum pimpinellifolium L3708) and tomato susceptible to P. infestans (S. lycopersicum Zaofen No. 2). Our results have, therefore, demonstrated that miR482b is an important component of defense response network. This will also help to identify candidate genes involved in plant–pathogen interaction.


MiR482b Overexpressing Phytophthora infestans RNA-Seq Tomato Regulatory network 



1-Aminocyclopropane-1-carboxylic acid


1-Aminocyclopropane-1-carboxylic acid synthase


Allene oxide synthase




Differentially expressed genes




Effector-triggered immunity


Fragments per kilobase of exon model per Million mapped reads


Gene ontology


Hypersensitive response


Jasmonic acid


Kyoto encyclopedia of genes and genomes


Linoleate 13S-lipoxygenase


OPC-8:0 CoA ligase 1


Pathogen/microbe-associated molecular patterns


Pattern-recognition receptors


PAMP-triggered immunity


Respiratory burst oxidase


Reactive oxygen species


Salicylic acid




S-Adenosylmethionine synthetase



The authors thank Prof. Weixing Shan (Northwest A&F University of China) for providing Phytophthora infestans. This work was supported by grants from the National Natural Science Foundation of China (Nos. 31471880 and 61472061).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

299_2018_2344_MOESM1_ESM.tif (390 kb)
Fig. S1 Experiment design in this study (TIF 390 KB)
299_2018_2344_MOESM2_ESM.tif (59 kb)
Fig. S2 Expression level of miR482b in the S. lycopersicum Zaofen No. 2 at 3 dpi. Actin expression was used as a control. Data are the means ± SEs of three independent experiments. Letters indicate significant differences among samples, and letters shared in common between or among the groups indicate no significant difference at the P < 0.05 level (TIF 59 KB)
299_2018_2344_MOESM3_ESM.tif (552 kb)
Fig. S3 Differentially expressed genes identified by RNA-Seq and validated by qRT-PCR. SlPOD, peroxidase (Solyc11g018800.1); SlCCoAOMT, caffeoyl-CoA O-methyltransferase (Solyc02g093230.2); Sl4CL, 4-coumarate--CoA ligase (Solyc03g097030.2); SlPAL, phenylalanine ammonia-lyase (Solyc05g056170.2). The Y-axis represents normalized relative expression values. The samples are labeled along the X-axis. Actin expression was used as a control. Data are the means ± SEs of three independent experiments. Letters indicate significant differences among samples, and letters shared in common between or among the groups indicate no significant difference at the P < 0.05 level (TIF 552 KB)
299_2018_2344_MOESM4_ESM.docx (16 kb)
Supplementary material 4 (DOCX 15 KB)
299_2018_2344_MOESM5_ESM.xlsx (46 kb)
Supplementary material 5 (XLSX 45 KB)
299_2018_2344_MOESM6_ESM.docx (27 kb)
Supplementary material 6 (DOCX 27 KB)
299_2018_2344_MOESM7_ESM.docx (16 kb)
Supplementary material 7 (DOCX 15 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Ning Jiang
    • 1
  • Jun Cui
    • 1
  • Guanglei Yang
    • 1
  • Xiaoli He
    • 1
  • Jun Meng
    • 2
  • Yushi Luan
    • 1
    Email author
  1. 1.School of Life Science and BiotechnologyDalian University of TechnologyDalianChina
  2. 2.School of Computer Science and TechnologyDalian University of TechnologyDalianChina

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