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Plant Cell Reports

, Volume 37, Issue 12, pp 1653–1666 | Cite as

Triticum urartu MTP1: its ability to maintain Zn2+ and Co2+ homeostasis and metal selectivity determinants

  • Fan-Hong Wang
  • Kun Qiao
  • Shuang Liang
  • Si-Qi Tian
  • Yan-Bao Tian
  • Hong Wang
  • Tuan-Yao Chai
Original Article

Abstract

Key message

TuMTP1 maintains Zn2+ and Co2+ homeostasis by sequestering excess Zn2+ and Co2+ into vacuoles. The mutations NSEDD/VTVTT in the His-rich loop and I119F in TMD3 of TuMTP1 restrict metal selectivity.

Abstract

Mineral nutrients, such as zinc (Zn) and cobalt (Co), are essential or beneficial for plants but can be toxic at elevated levels. Metal tolerance proteins (MTPs) are plant members of the cation diffusion facilitator (CDF) transporter family involved in cellular metal homeostasis. However, the determinants of substrate selectivity have not been clarified due to the diversity of MTP1 substrates in various plants. In this study, Triticum urartu MTP1 was characterized. When expressed in yeast, TuMTP1 conferred tolerance to Zn2+ and Co2+ but not Fe2+, Cu2+, Ni2+ or Cd2+ in solid and liquid culture and localized on the vacuolar membrane. Furthermore, TuMTP1-expressing yeast accumulated more Zn2+ and Co2+ when treated. TuMTP1 expression in T. urartu roots was significantly increased under Zn2+ and Co2+ stresses. Determinants of substrate selectivity were then examined through site-directed mutagenesis. The exchange of NSEDD with VTVTT in the His-rich loop of TuMTP1 restricted its metal selectivity to Zn2+, whereas the I119F mutation confined specificity to Co2+. The mutations H74, D78, H268 and D272 (in the Zn2+-binding site) and Leu322 (in the C-terminal Leu-zipper) partially or completely abolished the transport function of TuMTP1. These results show that TuMTP1 might sequester excess cytosolic Zn2+ and Co2+ into yeast vacuoles to maintain Zn2+ and Co2+ homeostasis. The NSEDD/VTVTT and I119F mutations are crucially important for restricting the substrate specificity of TuMTP1, and the Zn2+-binding site and Leu322 are essential for its ion selectivity and transport function. These results can be employed to change metal selectivity for biofortification or phytoremediation applications.

Keywords

Triticum urartu CDF TuMTP1 Zn2+ Co2+ Metal selectivity 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant no. C31370281, Grant no. U1632111, Grant no. 61672489), the Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences (Grant no. Y4ZK111B01), and the Chinese Academy of Sciences (Grant No. KJRH2015-001).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Life SciencesUniversity of Chinese Academy of SciencesBeijingChina
  2. 2.Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Engineering Research Center for Marine Algal Biotechnology, College of Life Science and OceanographyShenzhen UniversityShenzhenChina
  3. 3.Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina
  4. 4.The Innovative Academy of Seed DesignChinese Academy of ScienceBeijingChina
  5. 5.Southeast Asia Biodiversity Research InstituteChinese Academy of SciencesNay Pyi TawMyanmar

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