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Plant Cell Reports

, Volume 37, Issue 11, pp 1499–1511 | Cite as

AtDIV2, an R-R-type MYB transcription factor of Arabidopsis, negatively regulates salt stress by modulating ABA signaling

  • Qing Fang
  • Qiong Wang
  • Hui Mao
  • Jing Xu
  • Ying Wang
  • Hao Hu
  • Shuai He
  • Junchu Tu
  • Chao Cheng
  • Guozheng Tian
  • Xianqiang Wang
  • Xiaopeng Liu
  • Chi Zhang
  • Keming Luo
Original Article

Abstract

Key message

AtDIV2 integrates ABA signaling to negatively regulate salt stress in Arabidopsis.

Abstract

AmDIV (DIVARICATA) is a functional MYB transcription factor (TF) that regulates ventral identity during floral development in Antirrhinum. There are six members of DIV homologs in Arabidopsis; however, the functions of these proteins are largely unknown. Here, we characterized an R-R-type MYB TF AtDIV2, which is involved in salt stress responses and abscisic acid (ABA) signaling. Although universally expressed in tissues, the nuclear-localized AtDIV2 appeared not to be involved in seedling development processes. However, upon exposure to salt stress and exogenous ABA, the transcripts of AtDIV2 are markedly increased in wild-type (Wt) plants. The loss-of-function mutant div2 displayed much more tolerance to salt stress, and several salt-responsive genes were up-regulated. In addition, the div2 mutant showed higher sensitivity to ABA during seed germination. And the germination variance between the Wt and div2 mutant cannot be rectified by treatment with both ABA and sodium tungstate at the same time. ELISA results showed that the endogenous ABA content in the div2 mutant is clearly increased than that in Wt plants. Furthermore, the transcriptional expressions of several ABA-related genes, including ABA1 and ABI3, were elevated. Taken together, our results suggest that the R-R-type MYB TF AtDIV2 plays negative roles in salt stress and is required for ABA signaling in Arabidopsis.

Keywords

DIV Salt stress MYB Transcription factor ABA 

Notes

Acknowledgements

The present research is financially supported by National Natural Science Foundation of China (31660186) and China Postdoctoral Science Foundation funded project (2013M531923), and the Innovative training Program for College students of Hubei (201510517024).

Compliance with ethical standards

Conflict of interest

The authors have declared that no conflict of interest exists.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Qing Fang
    • 1
  • Qiong Wang
    • 1
  • Hui Mao
    • 1
  • Jing Xu
    • 1
  • Ying Wang
    • 1
  • Hao Hu
    • 1
  • Shuai He
    • 1
  • Junchu Tu
    • 1
  • Chao Cheng
    • 1
  • Guozheng Tian
    • 1
  • Xianqiang Wang
    • 3
  • Xiaopeng Liu
    • 1
  • Chi Zhang
    • 1
  • Keming Luo
    • 2
    • 3
  1. 1.Key Laboratory of Biological Resources Protection and Utilization of Hubei ProvinceHubei University for NationalitiesEnshiChina
  2. 2.Key Laboratory of Adaptation and Evolution of Plateau BiotaNorthwest Institute of Plateau Biology, Chinese Academy of SciencesXiningChina
  3. 3.Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Institute of Resources Botany, School of Life Sciences, Ministry of Education ChongqingSouthwest UniversityChongqingChina

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