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Plant Cell Reports

, Volume 37, Issue 8, pp 1113–1125 | Cite as

Isolation, characterization, and evaluation of three Citrus sinensis-derived constitutive gene promoters

  • L. Erpen
  • E. C. R. Tavano
  • R. Harakava
  • M. Dutt
  • J. W. Grosser
  • S. M. S. Piedade
  • B. M. J. Mendes
  • F. A. A. Mourão FilhoEmail author
Original Article

Abstract

Key message

Regulatory sequences from the citrus constitutive genes cyclophilin (CsCYP), glyceraldehyde-3-phosphate dehydrogenase C2 (CsGAPC2), and elongation factor 1-alpha (CsEF1) were isolated, fused to the uidA gene, and qualitatively and quantitatively evaluated in transgenic sweet orange plants.

Abstract

The 5′ upstream region of a gene (the promoter) is the most important component for the initiation and regulation of gene transcription of both native genes and transgenes in plants. The isolation and characterization of gene regulatory sequences are essential to the development of intragenic or cisgenic genetic manipulation strategies, which imply the use of genetic material from the same species or from closely related species. We describe herein the isolation and evaluation of the promoter sequence from three constitutively expressed citrus genes: cyclophilin (CsCYP), glyceraldehyde-3-phosphate dehydrogenase C2 (CsGAPC2), and elongation factor 1-alpha (CsEF1). The functionality of the promoters was confirmed by a histochemical GUS assay in leaves, stems, and roots of stably transformed citrus plants expressing the promoter-uidA construct. Lower uidA mRNA levels were detected when the transgene was under the control of citrus promoters as compared to the expression under the control of the CaMV35S promoter. The association of the uidA gene with the citrus-derived promoters resulted in mRNA levels of up to 60–41.8% of the value obtained with the construct containing CaMV35S driving the uidA gene. Moreover, a lower inter-individual variability in transgene expression was observed amongst the different transgenic lines, where gene constructs containing citrus-derived promoters were used. In silico analysis of the citrus-derived promoter sequences revealed that their activity may be controlled by several putative cis-regulatory elements. These citrus promoters will expand the availability of regulatory sequences for driving gene expression in citrus gene-modification programs.

Keywords

Cis-regulatory elements Cultivar improvement Gene expression Sweet orange 

Notes

Acknowledgements

LE and ECRT acknowledge Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for research financial support and fellowships. BMJM, FAAMF, and LE acknowledge Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for research fellowships.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

299_2018_2298_MOESM1_ESM.docx (22 kb)
Supplementary material 1 (DOCX 23 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • L. Erpen
    • 1
  • E. C. R. Tavano
    • 2
  • R. Harakava
    • 3
  • M. Dutt
    • 4
  • J. W. Grosser
    • 4
  • S. M. S. Piedade
    • 1
  • B. M. J. Mendes
    • 2
  • F. A. A. Mourão Filho
    • 1
    Email author
  1. 1.Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São PauloPiracicabaBrazil
  2. 2.Centro de Energia Nuclear na Agricultura, Universidade de São PauloPiracicabaBrazil
  3. 3.Instituto BiológicoSão PauloBrazil
  4. 4.Citrus Research and Education CenterUniversity of FloridaLake AlfredUSA

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