Plant Cell Reports

, Volume 37, Issue 6, pp 873–885 | Cite as

Evaluation of the plant growth-promoting activity of Pseudomonas nitroreducens in Arabidopsis thaliana and Lactuca sativa

  • Cao Son Trinh
  • Hyeri Lee
  • Won Je Lee
  • Seok Jin Lee
  • Namhyun Chung
  • Juhyeong Han
  • Jongyun Kim
  • Suk-Whan HongEmail author
  • Hojoung LeeEmail author
Original Article


Key message

Pseudomonas nitroreducens: strain IHB B 13561 (PnIHB) enhances the growth of Arabidopsis thaliana and Lactuca sativa via the stimulation of cell development and nitrate absorption.


Plant growth-promoting rhizobacteria (PGPR) enhance plant development through various mechanisms; they improve the uptake of soil resources by plants to greatly promote plant growth. Here, we used Arabidopsis thaliana seedlings and Lactuca sativa to screen the growth enhancement activities of a purified PGPR, Pseudomonas nitroreducens strain IHB B 13561 (PnIHB). When cocultivated with PnIHB, both species of plants exhibited notably improved growth, particularly in regard to biomass. Quantitative reverse transcription polymerase chain reaction analysis indicated high expression levels of the nitrate transporter genes, especially NRT2.1, which plays a major role in the high-affinity nitrate transport system in roots. Moreover, enhanced activity of the cyclin-B1 promoter was observed when wild-type ‘Columbia-0’ Arabidopsis seedlings were exposed to PnIHB, whereas upregulation of cyclin-B also occurred in the inoculated lettuce seedlings. Overall, these results suggest that PnIHB improves A. thaliana and L. sativa growth via specific pathways involved in the promotion of cell development and enhancement of nitrate uptake.


Arabidopsis thaliana CycB1 Lactuca sativa PGPR PnIHB NRT2 



Financial support for this work was provided through a grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (to H.L., 2016; Grant #2016-116118-3).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

299_2018_2275_MOESM1_ESM.pdf (638 kb)
Supplementary material 1 (PDF 638 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Cao Son Trinh
    • 1
  • Hyeri Lee
    • 1
  • Won Je Lee
    • 1
  • Seok Jin Lee
    • 1
  • Namhyun Chung
    • 1
  • Juhyeong Han
    • 2
  • Jongyun Kim
    • 1
  • Suk-Whan Hong
    • 3
    Email author
  • Hojoung Lee
    • 1
    Email author
  1. 1.Department of Biosystems and Biotechnology, College of Life Sciences and BiotechnologyKorea UniversitySeoulRepublic of Korea
  2. 2.Odus R&D CenterEumseong-GunRepublic of Korea
  3. 3.Department of Molecular Biotechnology, College of Agriculture and Life Sciences, Bioenergy Research CenterChonnam National UniversityGwangjuRepublic of Korea

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