Plant Cell Reports

, Volume 36, Issue 12, pp 1929–1942 | Cite as

ThPP1 gene, encodes an inorganic pyrophosphatase in Thellungiella halophila, enhanced the tolerance of the transgenic rice to alkali stress

  • Rui He
  • Guohong Yu
  • Xiaori Han
  • Jiao Han
  • Wei Li
  • Bing Wang
  • Shengcai Huang
  • Xianguo Cheng
Original Article


Key message

An inorganic pyrophosphorylase gene, ThPP1 , modulated the accumulations of phosphate and osmolytes by up-regulating the differentially expression genes, thus enhancing the tolerance of the transgenic rice to alkali stress (AS).


Inorganic pyrophosphorylase is essential in catalyzing the hydrolysis of pyrophosphate to inorganic phosphate during plant growth. Here, we report the changes of physiological osmolytes and differentially expression genes in the transgenic rice overexpressing a soluble inorganic pyrophosphatase gene ThPP1 of Thellungiella halophila in response to AS. Analyses showed that the ThPP1 gene was a PPase family I member which is located to the cytoplasm. Data showed that the transgenic lines revealed an enhanced tolerance to AS compared to the wild type, and effectively increased the accumulations of inorganic phosphate and organic small molecules starch, sucrose, proline and chlorophyll, and maintained the balance of osmotic potential by modulating the ratio of Na+/K+ in plant cells. Under AS, total 379 of differentially expression genes were up-regulated in the leaves of the transgenic line compared with control, and the enhanced tolerance of the transgenic rice to the AS seemed to be associated with the up-regulations of the osmotic stress-related genes such as the L-type lectin-domain containing receptor kinase (L-type LecRK), the cation/H+ antiporter gene and the vacuolar cation/proton exchanger 1 gene (CAX1), which conferred the involvements in the biosynthesis and metabolic pathways. Protein interaction showed that the ThPP1 protein specifically interacted with a 16# target partner of the photosystem II light-harvesting-Chl-binding protein. This study suggested that the ThPP1 gene plays an important regulatory role in conferring the tolerance of the transgenic rice to AS, and is an effective candidate in molecular breeding for crop cultivation of the alkali tolerance.


Alkali stress Gene expression Inorganic pyrophosphatase ThPP1 gene Rice Transcriptome sequencing 



We are grateful to Prof. Youzhi Ma and Prof. Ming Chen for providing the S. cerevisiae NMY51 and technical support. This work was supported by the National Key Project for Cultivation of New Varieties of Genetically Modifying Organisms (2016ZX08002-005) and the National Key Project for 973 Fundamental Research (2015CB150800). Special thanks to Prof. Wan Jianmin’s team for rice transformation.

Compliance with ethical standards

Conflict of interest

All authors have agreed on the content of the manuscript and declare no conflicts of interests.

Supplementary material

299_2017_2208_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 17 kb)


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Rui He
    • 1
    • 2
  • Guohong Yu
    • 2
  • Xiaori Han
    • 1
  • Jiao Han
    • 2
    • 3
  • Wei Li
    • 2
  • Bing Wang
    • 2
  • Shengcai Huang
    • 2
  • Xianguo Cheng
    • 2
  1. 1.College of Land and EnvironmentShenyang Agricultural UniversityShenyangPeople’s Republic of China
  2. 2.Key Lab of Plant Nutrition and Fertilizers, Ministry of Agriculture, Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  3. 3.College of Life ScienceShanxi Normal UniversityLinfenPeople’s Republic of China

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