GR1-like gene expression in Lycium chinense was regulated by cadmium-induced endogenous jasmonic acids accumulation
- 277 Downloads
The G1-like gene from the Lycium chinense was cloned and transferred into N. tabacum. Evidence showed that endogenous JA accumulation was crucial to LcGR gene expression in cadmium-stressed L. chinense.
Glutathione reductase (GR) plays a vital role in glutathione–ascorbate metabolism and is a key enzyme in maintaining the redox state in plants. Jasmonic acids (JA) are important hormones regulating protective responses against bacteria and mechanic damage in plants. At present, the relationship between the endogenous JA accumulation, the glutathione (GSH) content and GR gene expression in plants under cadmium (Cd) stress has not been elucidated. This study primarily aims to explore their interconnected relations. First, we isolated the GR1-like gene from Lycium chinense (LcGR). Real-time PCR showed that gene LcGR and allene oxide cyclase (LcAOC) (a JA synthesis gene) expression in L. chinense plants was significantly enhanced by CdCl2 and reduced by CdCl2 cotreatment with 12,13-epoxy-octadecenoic acid (EOA), a JA synthesis inhibitor. Meanwhile, the JA content in plants strongly increased under Cd stress and decreased under Cd + EOA treatment, which was in accordance with expression pattern of LcAOC. The function of gene LcGR was confirmed in vitro with E. coli expression system. The subcellular localization in chloroplasts of LcGR gene was proved in Nicotiana tabacum leaves with transient transfection system of Agrobacterium tumefaciens. Furthermore, the overexpression of gene LcGR in the transgenic tabacum led to great Cd-tolerance and higher GSH accumulation. Overall, the results showed that the endogenous JA accumulation in Cd-stressed plants affects the GR expression which is crucial to the GSH accumulation and GSH-dependent tolerance to cadmium in LcGR transformants.
KeywordsGlutathione reductase Lycium chinense Cadmium Jasmonic acids Oxidative stress
This subject is supported by the National Science and Technology Major Project of China on GMO Cultivation for New Variaties (No. 2014ZX0800302B), National Natural Science Foundation of China (Nos. 31271793 and 31271419), Tianjin Research Program of Application Foundation and Advanced Technology (No. 15JCQNJC14700).
Compliance with ethical standards
Conflict of interest
The authors have declared that they have no conflict of interest.
- Agrawal GK, Jwa NS, Agrawal SK, Tamogami S, Iwahashi H, Rakwal R (2003) Cloning of novel rice allene oxide cyclase (OsAOC): mRNA expression and comparative analysis with allene oxide synthase (OsAOS) gene provides insight into the transcriptional regulation of octadecanoid pathway biosynthetic genes in rice. Plant Sci 164(6):979–992CrossRefGoogle Scholar
- Ding S, Jiang R, Lu Q, Wen X, Lu C (2016) Glutathione reductase 2 maintains the function of photo- system II in Arabidopsis under excess light. Biochem Biophys Acta 6:665–677Google Scholar
- Foyer CH, Souriau N, Perret S, Lelandais M, Kunert KJ, Pruvost C, Jouanin L (1995) Overexpression of glutathione reductase but not glutathione synthetase leads to increases in antioxidant capacity and resistance to photoinhibition in poplar trees. Plant Physiol 109(3):1047–1057PubMedPubMedCentralCrossRefGoogle Scholar
- Le Martret B, Poage M, Shiel K, Nugent GD, Dix PJ (2011) Tobacco chloroplast transformants expressing genes encoding dehydroascorbate reductase, glutathione reductase, and glutathione-S-transferase, exhibit altered anti-oxidant metabolism and improved abiotic stress tolerance. Plant Biotechnol J 9(6):661–673PubMedCrossRefGoogle Scholar
- Mhamdi A, Hager J, Chaouch S, Queval G, Han Y, Taconnat L, Saindrenan P, Gouia H, Issakidis-Bourguet E, Renou JP, Noctor G (2010) Arabidopsis glutahione reductase 1 plays a crucial role in leaf responses to intracellular hydrogen peroxide and in ensuring appropriate gene expression through both salicylic acid and jasmonic acid signaling pathways. Plant Physiol 153(3):1144–1160PubMedPubMedCentralCrossRefGoogle Scholar
- Rodríguez-serrano M, Romero-puertas MC, Zabalza ANA, Corpas FJ, Gómez M, Del Rio LA, Sandalio LM (2006) Cadmium effect on oxidative metabolism of pea (Pisum sativum L.) roots. Imaging of reactive oxygen species and nitric oxide accumulation in vivo. Plant Cell Environ 29(8):1532–1544PubMedCrossRefGoogle Scholar
- Zaman T, Asaeda T (2014) Assessment of macro-micro element accumulation capabilities of Elodea nuttallii under gradient redox statuses with elevated NH4-N concentrations. Acta Bot Croat 73(1):131–147Google Scholar