Plant Cell Reports

, Volume 36, Issue 7, pp 1065–1082 | Cite as

Targeted gene disruption coupled with metabolic screen approach to uncover the LEAFY COTYLEDON1-LIKE4 (L1L4) function in tomato fruit metabolism

  • Custódia Gago
  • Victoria Drosou
  • Konstantinos Paschalidis
  • Adriana Guerreiro
  • Graça Miguel
  • Dulce Antunes
  • Zoe HiliotiEmail author
Original Article


Key message

Functional analysis of tomato L1L4 master transcription factor resulted in important metabolic changes affecting tomato fruit quality.


Tomato fruits from mutant lines bearing targeted disruption of the heterotrimeric nuclear transcription factor Y (NF-Y) transcription factor (TF) gene LEAFY-COTYLEDON1-LIKE4 (L1L4, NF-YB6), a master regulator of biosynthesis for seed storage proteins and fatty acids, were evaluated for metabolites content and morphology. Metabolic screens using LC–MS/MS-based analysis and physico-chemical methods in different L1L4 mutants of the fourth generation allowed a comparative assessment of the effects of the TF disruption. Mutagenesis resulted in fruits phenotypically similar to wild-type with subtle shape differences in the distal end protrusion and symmetry. Conversely, mutant fruits from independent lines had significant variation in moisture content, titratable acidity and overall metabolite profiles including oxalic and citric acid, fructose, β-carotene, total polyphenols and antioxidants. Lines 6, 7 and 9 were the richest in β-carotene and antioxidant activity, line 4 in ascorbic acid and lines 4 and 8 in succinic acid. The reduced content of the anti-nutrient oxalic acid in several mutant fruits suggests that L1L4 gene may regulate the accumulation of this compound during fruit development. Detailed LC–MS/MS analysis of mutant seeds showed substantial differences in bioactive compounds compared to wild-type seeds. Taken together, the results suggest that the L1L4 TF is a significant regulator of metabolites both in tomato fruit and seeds providing a molecular target for crop improvement. Elucidation of the candidate genes encoding key enzymes in the affected metabolic pathways aimed to facilitate the L1L4 gene network exploration and eventually lead to systems biology approaches in tomato fruit quality.


L1L4/NF-YB6 Fruit quality Metabolites Zinc finger nuclease technology Transcription factor Tomato 



This work was benefited from the networking activities within the European funded COST ACTION FA1106 Quality Fruit.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

299_2017_2137_MOESM1_ESM.docx (2.1 mb)
Supplementary material 1 (DOCX 2194 KB)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Custódia Gago
    • 1
  • Victoria Drosou
    • 2
  • Konstantinos Paschalidis
    • 3
  • Adriana Guerreiro
    • 1
  • Graça Miguel
    • 1
  • Dulce Antunes
    • 1
    • 4
  • Zoe Hilioti
    • 2
    Email author
  1. 1.Meditbio, FCTUniversity of AlgarveFaroPortugal
  2. 2.Institute of Applied BiosciencesCentre for Research and Technology HellasThessalonikiGreece
  3. 3.Department of AgricultureTechnological Educational Institution of CreteHeraklionGreece
  4. 4.CEOT, FCTUniversity of AlgarveFaroPortugal

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