Plant Cell Reports

, Volume 36, Issue 3, pp 419–435

Auxin synthesis gene tms1 driven by tuber-specific promoter alters hormonal status of transgenic potato plants and their responses to exogenous phytohormones

  • Oksana O. Kolachevskaya
  • Lidiya I. Sergeeva
  • Kristyna Floková
  • Irina A. Getman
  • Sergey N. Lomin
  • Valeriya V. Alekseeva
  • Elena B. Rukavtsova
  • Yaroslav I. Buryanov
  • Georgy A. Romanov
Original Article

DOI: 10.1007/s00299-016-2091-y

Cite this article as:
Kolachevskaya, O.O., Sergeeva, L.I., Floková, K. et al. Plant Cell Rep (2017) 36: 419. doi:10.1007/s00299-016-2091-y

Abstract

Key message

Ectopic auxin overproduction in transgenic potato leads to enhanced productivity accompanied with concerted and occasional changes in hormonal status, and causing altered response of transformants to exogenous auxin or cytokinin.

Abstract

Previously, we generated potato transformants expressing Agrobacterium-derived auxin synthesis gene tms1 driven by tuber-specific patatin gene promoter (B33-promoter). Here, we studied the endogenous hormonal status and the response to exogenous phytohormones in tms1 transformants cultured in vitro. Adding indole-3-acetic acid (IAA) or kinetin to culture medium affected differently tuberization of tms1-transformed and control plants, depending also on sucrose content in the medium. Exogenous phytohormones ceased to stimulate the tuber initiation in transformants at high (5–8%) sucrose concentration, while in control plants the stimulation was observed in all experimental settings. Furthermore, exogenous auxin partly inhibited the tuber initiation, and exogenous cytokinin reduced the average tuber weight in most transformants at high sucrose content. The elevated auxin level in tubers of the transformants was accompanied with a decrease in content of cytokinin bases and their ribosides in tubers and most shoots. No concerted changes in contents of abscisic, jasmonic, salicylic acids and gibberellins in tubers were detected. The data on hormonal status indicated that the enhanced productivity of tms1 transformants was due to auxin and not mediated by other phytohormones. In addition, exogenous cytokinin was shown to upregulate the expression of genes encoding orthologs of auxin receptors. Overall, the results showed that tms1 expression and local increase in IAA level in transformants affect both the balance of endogenous cytokinins and the dynamics of tuberization in response to exogenous hormones (auxin, cytokinin), the latter reaction depending also on the carbohydrate supply. We introduce a basic model for the hormonal network controlling tuberization.

Keywords

Tuberization Potato Auxin Cytokinin Phytohormones Transformants Hormonal status Solanum tuberosum 

Supplementary material

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Supplementary material 1 (DOCX 1405 kb)
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Supplementary material 5 (DOCX 15 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Oksana O. Kolachevskaya
    • 1
  • Lidiya I. Sergeeva
    • 2
  • Kristyna Floková
    • 2
  • Irina A. Getman
    • 1
  • Sergey N. Lomin
    • 1
  • Valeriya V. Alekseeva
    • 3
  • Elena B. Rukavtsova
    • 3
  • Yaroslav I. Buryanov
    • 3
  • Georgy A. Romanov
    • 1
    • 4
  1. 1.Laboratory of Signaling SystemsInstitute of Plant Physiology RASMoscowRussia
  2. 2.Laboratory of Plant PhysiologyWageningen UniversityWageningenThe Netherlands
  3. 3.Plant Biotechnology LaboratoryBranch of Institute of Bioorganic Chemistry RASPushchinoRussia
  4. 4.Belozersky Institute of Physico-Chemical BiologyMoscow State UniversityMoscowRussia