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Plant Cell Reports

, Volume 36, Issue 1, pp 89–102 | Cite as

LcMCII-1 is involved in the ROS-dependent senescence of the rudimentary leaves of Litchi chinensis

  • Congcong Wang
  • Peitao Lü
  • Silin Zhong
  • Houbin Chen
  • Biyan ZhouEmail author
Original Article

Abstract

Key message

LcMCII - 1 is a type II metacaspase. Over-expression of LcMCII- 1 in Arabidopsis promoted ROS-dependent and natural senescence. Virus-induced LcMCII- 1 silencing delayed the ROS-dependent senescence of the rudimentary leaves of Litchi chinensis .

Abstract

Litchi is an evergreen woody fruit tree that is widely cultivated in subtropical and tropical regions. Its floral buds are mixed with axillary or apical panicle primordia, leaf primordia and rudimentary leaves. A low spring temperature is vital for litchi production as it promotes the abscission of the rudimentary leaves, which could otherwise prevent panicle development. Hence, climate change could present additional challenges for litchi production. We previously reported that reactive oxygen species (ROS) can substitute low-temperature treatment to induce the senescence of rudimentary leaves. We have now identified from RNA-Seq data a litchi type II metacaspase gene, LcMCII-1, that is responsive to ROS. Silencing LcMCII-1 by virus-induced gene silencing delayed ROS-dependent senescence. The ectopic over-expression of LcMCII-1 in transgenic Arabidopsis promoted ROS-dependent and natural senescence. Consistently, the transient expression of LcMCII-1 in tobacco leaf by agroinfiltration resulted in leaf yellowing. Our findings demonstrate that LcMCII-1 is positively involved in the regulation of rudimentary leaf senescence in litchi and provide a new target for the future molecular breeding of new cultivars that can set fruit in warmer climates.

Keywords

Rudimentary leaf Senescence Metacaspase Litchi VIGS Reactive oxygen species 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation (project no. 31071760) and the China Agricultural Industry Project (project no. CARS-33-08).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

299_2016_2059_MOESM1_ESM.pdf (7.7 mb)
Supplementary material 1 (PDF 7837 kb)
299_2016_2059_MOESM2_ESM.doc (42 kb)
Supplementary material 2 (DOC 41 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Congcong Wang
    • 1
  • Peitao Lü
    • 2
  • Silin Zhong
    • 2
  • Houbin Chen
    • 1
  • Biyan Zhou
    • 1
    Email author
  1. 1.Guangdong Litchi Engineering Research Center, College of HorticultureSouth China Agricultural UniversityGuangzhouChina
  2. 2.State Key Laboratory of Agrobiotechnology, School of Life SciencesChinese University of Hong KongHong KongChina

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