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Plant Cell Reports

, Volume 35, Issue 3, pp 613–627 | Cite as

CarNAC4, a NAC-type chickpea transcription factor conferring enhanced drought and salt stress tolerances in Arabidopsis

  • Xingwang Yu
  • Yanmin Liu
  • Shuang Wang
  • Yuan Tao
  • Zhankui Wang
  • Yingjie Shu
  • Hui Peng
  • Abudoukeyumu Mijiti
  • Ze Wang
  • Hua Zhang
  • Hao MaEmail author
Original Article

Abstract

Key message

CarNAC4 is a typical stress-responsive NAC transcription factor and enhances drought and salt stress tolerances in transgenic Arabidopsis.

Abstract

Chickpea (Cicer arietinum L.) is relatively vulnerable to abiotic stress conditions, but the tolerance mechanisms for such stresses in chickpea are largely unknown. To identify stress-related factors in chickpea, we previously constructed a cDNA library of chickpea leaves exposed to drought stress conditions. A cDNA encoding a putative NAC transcription factor (CarNAC4) was identified as a putative stress-responsive gene. Our study indicated that the transcript levels of CarNAC4 were enhanced in response to several abiotic stresses and phytohormones. Promoter analysis demonstrated that multiple stress-related cis-acting elements exist in promoter region of CarNAC4. CarNAC4 is localized in the nucleus and binds to the DNA sequence containing CGT[G/A], while the C-terminal region of CarNAC4 contains a transcriptional activation domain. Over-expression of CarNAC4 in Arabidopsis plants improved tolerance to drought and salt stresses. Transgenic plants exhibited greater reduced rates of water loss and more proline accumulation than Col-0 plants under drought stress and less MDA contents than Col-0 plants under salt stress. In addition, over-expression of CarNAC4 enhanced the expression of stress-responsive genes such as RD29A, ERD10, COR15A, COR47, KIN1 and DREB2A. These results indicated that CarNAC4 functions as a transcription factor involved in the regulation of drought and salt stress response.

Keywords

NAC transcription factor Stress tolerance Transgenic plants Chickpea 

Abbreviations

TF

Transcription factor

DRE

Dehydration responsive element

LTRE

Low temperature-responsive element

ABRE

ABA-responsive elements

MDA

Malondialdehyde

ORF

Open reading frame

P

Probe

PM

Mutant probe

qRT-PCR

Quantitative reverse transcriptase PCR

RACE

Rapid amplification of cDNA ends

TSS

Transcriptional start site

Notes

Acknowledgments

We gratefully acknowledge the partial financial support from the projects supported by the National Natural Science Foundation of China (31160306 and 30860152), and from the project supported by the Xinjiang Science and Technology Department of China (200991254) for this research.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Supplementary material

299_2015_1907_MOESM1_ESM.docx (1.1 mb)
Supplementary material 1 (DOCX 1144 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Xingwang Yu
    • 1
  • Yanmin Liu
    • 1
  • Shuang Wang
    • 1
  • Yuan Tao
    • 1
  • Zhankui Wang
    • 1
  • Yingjie Shu
    • 1
  • Hui Peng
    • 2
    • 3
  • Abudoukeyumu Mijiti
    • 4
  • Ze Wang
    • 4
  • Hua Zhang
    • 4
  • Hao Ma
    • 1
    • 4
    Email author
  1. 1.State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingChina
  2. 2.Key Laboratory of Ecology of Rare and Endangered Species and Environmental ProtectionGuangxi Normal University, Ministry of EducationGuilinChina
  3. 3.College of Life SciencesGuangxi Normal UniversityGuilinChina
  4. 4.Institute of Desert in the Arid Areas, College of Grassland and Environment SciencesXinjiang Agricultural UniversityUrumqiChina

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