Molecular cloning and potential function prediction of homologous SOC1 genes in tree peony
- 585 Downloads
The central flower integrator PsSOC1 was isolated and its expression profiles were analyzed; then the potential function of PsSOC1 in tree peony was postulated.
The six flowering genes PrSOC1, PdSOC1, PsSOC1, PsSOC1-1, PsSOC1-2, and PsSOC1-3 were isolated from Paeonia rockii, Paeonia delavayi, and Paeonia suffruticosa, respectively. Sequence comparison analysis showed that the six genes were highly conserved and shared 99.41 % nucleotide identity. Further investigation suggested PsSOC1 was highly homologous to the floral integrators, SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1), from Arabidopsis. Phylogenetic analysis showed that the SOC1 protein clustering has family specificity and PsSOC1 has a close relationship with homologous SOC1 from Asteraceae species. The studies of PsSOC1’s expression patterns in different buds and flower buds, and vegetative organs indicated that PsSOC1 could express in both vegetative and reproductive organs. While the expression of PsSOC1 in different developmental stages of buds was different; high expression levels of PsSOC1 occurred in the bud at the bud sprouting stage and the type I aborted the flower bud. PsSOC1 expression was also shown to be affected by gibberellins (GA), low temperature, and photoperiod. One of the pathways that regulates tree peony flowering may be the GA-inductive pathway. Ectopic expression of PsSOC1 in tobacco demonstrated that greater PsSOC1 expression in the transgenic tobacco plants not only promoted plant growth, but also advanced the flowering time. Finally, the potential function of PsSOC1 in tree peony was postulated.
KeywordsTree peony MADS-box gene PsSOC1 Flowering time
Tobacco seeds (Nicotiana tabacum L. cv. Wisconis38) and the pCAMBIA2301G vector were kindly provided by Professor Mingyang Li (College of Horticulture and Landscape Architecture, Southwest University). This research was financially supported by grants from the National 863 plans projects (2011AA10020703), the Agricultural Science and Technology Innovation Program (ASTIP) of the Chinese Academy of Agricultural Sciences (2014–2015), the Special Fund for Agro-scientific Research in the Public Interest (201203071), and the Foundation of Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (2014JB02-001).
Conflict of interest
The authors declare that they have no conflict of interest.
- Ferrario S, Busscher J, Franken J, Gerats T, Vandenbussche M, Angenent GC, Immink RG (2004) Ectopic expression of the petunia MADS box gene UNSHAVEN accelerates flowering and confers leaf-like characteristics to floral organs in a dominant-negative manner. Plant Cell 16:1490–1505PubMedCentralPubMedCrossRefGoogle Scholar
- Heuer S, Hansen S, Bantin J, Brettschneider R, Kranz E, Lorz H, Dresselhas T (2001) The maize MADS box gene ZmMADS3 affects node number and spikelet development and is co-expressed with ZmMADS1 during flower development, in egg cells, and early embryogenesis. Plant Physiol 127:33–45PubMedCentralPubMedCrossRefGoogle Scholar
- Immink RGH, Posé D, Ferrario S, Ott F, Kaufmann K, Valentim FL, de Folter S, van der Wal F, van Dijk ADJ, Schmid M, Angenent GC (2012) Characterization of SOC1’s central role in flowering by the identification of its upstream and downstream regulators. Plant Physiol 160:433–449PubMedCentralPubMedCrossRefGoogle Scholar
- Li T, Niki T, Nishijima T, Douzono M, Koshioka M, Hisamatsu T (2009) Roles of CmFL, CmAFL1, and CmSOC1 in the transition from vegetative to reproductive growth in Chrysanthemum morifolium Ramat. J Hortic Sci Biotech 84:447–453Google Scholar
- Liu ZA (2003) Study on the forcing and retardation culture of tree peony (in Japanese with English summary). PhD thesis, Shimane University, Matue, pp 1–6Google Scholar
- Stern FC (1946) A study of the genus Paeonia. The Royal Hort Soc, London, pp 1–146Google Scholar
- Wang SL, Xue JQ, Zhu FY, Zhang P, Ren XX, Liu CJ, Zhang XX (2014b) Molecular cloning, expression and evolutionary analysis of the flowering-regulating transcription factor gene PsCOL4 in tree peony. Acta Hortic Sinica 41:1409–1417Google Scholar
- Wister JC (1928) the mountain tree peony. In: Boyd J (ed) Peonies. American Horticultural Society, Washington, pp 219–244Google Scholar