Plant Cell Reports

, Volume 34, Issue 2, pp 189–198 | Cite as

Expression differences of anthocyanin biosynthesis genes reveal regulation patterns for red pear coloration

  • Ya-nan Yang
  • Gai-fang Yao
  • Danman Zheng
  • Shao-ling Zhang
  • Chao Wang
  • Ming-yue Zhang
  • Jun Wu
Original Paper

Abstract

Key message

This research reveals the different expression patterns of anthocyanin biosynthesis enzyme genes and transcription factors in six red-skinned pear cultivars with different genetic backgrounds.

Abstract

Skin color is an important feature of pear fruits, with red skin generally attracting consumers. However, great differences of coloration exist in different pear cultivars. To elucidate the characteristics of pigmentation in pear cultivars with different genetic backgrounds, six cultivars, belonging to P. communis, P. pyrifolia, P. ussuriensis, P. bretschneideri, and a hybrid of P. communis × P. pyrifolia, were used to detect pigment concentrations, expressions of seven anthocyanin biosynthesis enzyme genes, and three related transcription factor genes. Results showed that the occidental pears ‘Starkrimson’ and ‘Red Bartlett’ colored at the beginning of fruit setting, but color decreased with fruit maturity; the other four cultivars showed low anthocyanin accumulations and the contents increased during fruit development, but also decreased at later stages. The expression patterns of genes encoding enzymes indicated that ANS and UFGT were decisive genes for anthocyanin biosynthesis for red-skinned pear, and their different expressions led to the coloration differences between occidental and oriental pears. The expression patterns of transcription factors indicated that the different co-expression of MYB10 and bHLH33 genes and the different expressions of WD40 are involved in the differential regulation mechanisms of anthocyanin biosynthesis and coloration pattern between occidental and oriental pears.

Keywords

Pear Red color Anthocyanin Gene expression 

Abbreviations

ANS

Anthocyanin synthase

bHLH

Basic helix loop helix

CHI

Chalcone isomerase

CHS

Chalcone synthase

DAFB

Days after full bloom

DFR

DihydroXavonol 4-reductase

DEPC

Diethylpyrocarbonate

F3H

Flavanone 3-hydroxylase

PAL

Phenylalanine ammonialyase

SE

Standard error

TF

Transcript factor

CT

Threshold cycle

UFGT

UDP-glucose: flavonoid-3-O-glucosyltransferase

qPCR

Quantitative reverse transcription polymerase chain reaction

Supplementary material

299_2014_1698_MOESM1_ESM.doc (109 kb)
Supplementary material 1 (DOC 109 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Ya-nan Yang
    • 1
  • Gai-fang Yao
    • 1
  • Danman Zheng
    • 2
  • Shao-ling Zhang
    • 1
  • Chao Wang
    • 1
  • Ming-yue Zhang
    • 1
  • Jun Wu
    • 1
  1. 1.Centre of Pear Engineering Technology ResearchNanjing Agricultural UniversityNanjingPeople’s Republic of China
  2. 2.Roy J. Carver Biotechnology CenterUniversity of Illinois Urbana-ChampaignUrbanaUSA

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