Plant Cell Reports

, Volume 34, Issue 1, pp 83–96 | Cite as

Differential regulation of proteins in rice (Oryza sativa L.) under iron deficiency

  • Lin Chen
  • Chengqiang Ding
  • Xiufeng Zhao
  • Junxu Xu
  • Alim Abdul Mohammad
  • Shaohua Wang
  • Yanfeng Ding
Original Paper

Abstract

Key message

Sixty-three proteins were identified to be differentially accumulated due to iron deficiency in shoot and root. The importance of these proteins alterations on shoot physiology is discussed.

Abstract

Iron (Fe) is an essential micronutrient for plant growth and its accumulation affects the quality of edible plant organs. To investigate the adaptive mechanism of a Chinese rice variety grown under iron deficiency, proteins differentially accumulated in leaves and roots of Yangdao 6, an indica cultivar, under Fe deficiency growth condition, were profiled using a two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS). The accumulations of seventy-three proteins were detected to be increased or decreased upon iron deficiency, and sixty-three of them were successfully identified. Among the sixty-three proteins, a total of forty proteins were identified in rice leaves, and twenty-three proteins were in roots. Most of these proteins are involved in photosynthesis, C metabolism, oxidative stress, Adenosine triphosphate synthesis, cell growth or signal transduction. The results provide a comprehensive way to understand, at the level of proteins, the adaptive mechanism used by rice shoots and roots under iron deficiency.

Keywords

Iron deficiency Proteomics Rice (Oryza sativa L.) Leaf Root Two-dimensional gel electrophoresis 

Notes

Acknowledgments

This work was supported by the Ministry of National Science and Technology of China (Project No. 2012BAD04B00) and the Science and Technology Department of Henan Province (Project No. 2013BAD07B00). We thank Professor Andre Jagendorf for the language editing.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Lin Chen
    • 1
  • Chengqiang Ding
    • 1
  • Xiufeng Zhao
    • 2
  • Junxu Xu
    • 1
  • Alim Abdul Mohammad
    • 3
  • Shaohua Wang
    • 1
  • Yanfeng Ding
    • 1
  1. 1.Key Laboratory of Crop Physiology and Ecology in Southern China, College of AgricultureMinistry of Agricultural University, Nanjing Agricultural UniversityNanjingChina
  2. 2.Institute of Agricultural Environment and ResourceShanxi Academy of Agricultural ScienceTaiyuanChina
  3. 3.Department of Agronomy and Agricultural ExtensionUniversity of RajshahiRajshahiBangladesh

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