Differential regulation of proteins in rice (Oryza sativa L.) under iron deficiency
- 679 Downloads
Sixty-three proteins were identified to be differentially accumulated due to iron deficiency in shoot and root. The importance of these proteins alterations on shoot physiology is discussed.
Iron (Fe) is an essential micronutrient for plant growth and its accumulation affects the quality of edible plant organs. To investigate the adaptive mechanism of a Chinese rice variety grown under iron deficiency, proteins differentially accumulated in leaves and roots of Yangdao 6, an indica cultivar, under Fe deficiency growth condition, were profiled using a two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS). The accumulations of seventy-three proteins were detected to be increased or decreased upon iron deficiency, and sixty-three of them were successfully identified. Among the sixty-three proteins, a total of forty proteins were identified in rice leaves, and twenty-three proteins were in roots. Most of these proteins are involved in photosynthesis, C metabolism, oxidative stress, Adenosine triphosphate synthesis, cell growth or signal transduction. The results provide a comprehensive way to understand, at the level of proteins, the adaptive mechanism used by rice shoots and roots under iron deficiency.
KeywordsIron deficiency Proteomics Rice (Oryza sativa L.) Leaf Root Two-dimensional gel electrophoresis
This work was supported by the Ministry of National Science and Technology of China (Project No. 2012BAD04B00) and the Science and Technology Department of Henan Province (Project No. 2013BAD07B00). We thank Professor Andre Jagendorf for the language editing.
Conflict of interest
The authors declare that they have no conflict of interest.
- Chatelain E, Hundertmark M, Leprince O, Le Gall S, Satour P, Deligny-Penninck S, Rogniaux H, Buitink J (2012) Temporal profiling of the heat-stable proteome during late maturation of Medicago truncatula seeds identifies a restricted subset of late embryogenesis abundant proteins associated with longevity. Plant Cell Environment 35:1440–1455CrossRefGoogle Scholar
- Chen Y, Barak P (1982) Iron nutrition of plants in calcareous soils. In: Brady NC (ed) Advance in agronomy. Rehovot, Israel, pp 217–240Google Scholar
- Guo D, Chen F, Inoue K, Blount JW, Dixon RA (2001) Downregulation of caffeic acid 3-O-methyltransferase and caffeoyl CoA 3-O-methyltransferase in transgenic alfalfa. impacts on lignin structure and implications for the biosynthesis of G and S lignin. Plant Cell 13:73–88PubMedCentralPubMedCrossRefGoogle Scholar
- Inoue H, Kobayashi T, Nozoye T, Takahashi M, Kakei Y, Suzuki K, Nakazono M, Nakanishi H, Mori S, Nishizawa NK (2009) Rice OsYSL15 is an Fe-regulated Fe(III)-deoxymugineic acid transporter expressed in the roots and is essential for Fe uptake in early growth of the seedlings. J Biol Chem 284:3470–3479PubMedCrossRefGoogle Scholar
- Nelson DL, Cox MM (2000) Lehninger. Principles of Biochemistry, New YorkGoogle Scholar
- Schönthal AH (1998) Role of PP2A in intracellular signal transduction pathways. Front Biosci 3:1262–1273Google Scholar
- Virshup DM, Cegielska A, Russo A, Kelly TJ, Shaffer S (1993) The initiation of SV40 DNA replication is controlled by a phosphorylation-dephosphorylation cycle. Adv Protein Phosphatases 7:271–293Google Scholar
- Yan JX, Wait R, Berkelman T, Harry RA, Westbrook JA, Wheeler CH, Dunn MJ (2000) A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry. Electrophoresis 21:3666–3672PubMedCrossRefGoogle Scholar
- Yoshida S, Forno DA, Cock JH, Gomez KA (1976) Laboratory manual for physiological studies of rice. Los BañosGoogle Scholar
- Zhao XF, Ding CQ, Chen L, Wang SH, Wang QS, Ding YF (2012) Comparative proteomic analysis of the effects of nitric oxide on alleviating Cd-induced toxicity in rice (Oryza sativa L.). Plant Omics J 5:604–614Google Scholar