Plant Cell Reports

, Volume 33, Issue 9, pp 1413–1423 | Cite as

In vitro conservation of Dendrobium germplasm

Review
First Online:
Received:
Revised:
Accepted:

Abstract

Dendrobium is a large genus in the family Orchidaceae that exhibits vast diversity in floral characteristics, which is of considerable importance to orchid breeders, biotechnologists and collectors. Native species have high value as a result of their medicinal properties, while their hybrids are important as ornamental commodities, either as cut flowers or potted plants and are thus veritable industrial crops. Thus, preservation of Dendrobium germplasm is valuable for species conservation, breeding programs and the floriculture industry. Cryopreservation represents the only safe, efficient and cost-effective long-term storage option to facilitate the conservation of genetic resources of plant species. This review highlights 16 years of literature related to the preservation of Dendrobium germplasm and comprises the most comprehensive assessment of thorough studies performed to date, which shows reliable and reproducible results. Air-drying, encapsulation–dehydration, encapsulation–vitrification, vitrification and droplet-vitrification are the current cryopreservation methodologies that have been used to cryopreserve Dendrobium germplasm. Mature seeds, pollen, protoplasts, shoot primordia, protocorms and somatic embryos or protocorm-like bodies (PLBs) have been cryopreserved with different levels of success. Encapsulation–vitrification and encapsulation–dehydration are the most used protocol, while PLBs represent the main explant explored.

Keywords

Cryopreservation Genetic breeding Micropropagation Orchidaceae Plant biotechnology Protocorm-like body 

Abbreviations

DMSO

Dimethyl sulfoxide

FDA

Fluorescein diacetate

LN

Liquid nitrogen

PLB

Protocorm-like body

TTC

Triphenyl tetrazolium chloride

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Notes

Conflict of interest

The authors declare no conflicts of interest, financial, or other.

References

  1. Antony JJJ, Keng CL, Rathinam X, Subramaniam S (2010) Preliminary study on cryopreservation of Dendrobium Bobby Messina protocorm-like bodies by vitrification technique. Afr J Biotechnol 9:7063–7070Google Scholar
  2. Antony JJJ, Keng CL, Rathinam X, Marimuthu S, Subramaniam S (2011a) Effect of preculture and PVS2 incubation conditions followed by histological analysis in cryopreserved PLBs of Dendrobium Bobby Messina. Aus J Crop Sci 5:1557–1564Google Scholar
  3. Antony JJJ, Sinniah UR, Keng CL, Pobathy R, Khoddamzadeh AA (2011b) Subramaniam S. Selected potential encapsulation–dehydration parameters on Dendrobium Bobby Messina protocorm-like bodies using TTC analysis. Aus J Crop Sci 5:1817–1822Google Scholar
  4. Antony JJJ, Poobathy R, Danial M, Sinniah UR, Subramaniam S (2012) Polymorphism analysis of cryopreserved Dendrobium Bobby Messina protocorm-like bodies (PLBs) using RAPD markers. Plant Omics J 5:427–431Google Scholar
  5. Antony JJJ, Keng CL, Mahmood M, Subramaniam S (2013) Effects of ascorbic acid on PVS2 cryopreservation of Dendrobium Bobby Messina’s PLBs supported with SEM analysis. Appl Biochem Biotechnol 171:315–329PubMedCrossRefGoogle Scholar
  6. Antony JJJ, Mubbarakh SA, Mahmood M, Subramaniam S (2014) Effect of plasmolysis on protocorm-like bodies of Dendrobium Bobby Messina orchid following cryopreservation with encapsulation–dehydration method. Appl Biochem Biotechnol 172:1433–1444PubMedCrossRefGoogle Scholar
  7. Bian H, Wang J, Lin W, Han M, Zhu M (2002) Accumulation of soluble sugars, heat-stable proteins and dehydrins in cryopreservation of protocorm-like bodies of Dendrobium candidum by air drying method. J Plant Physiol 153:1139–1145CrossRefGoogle Scholar
  8. Chen Y (2000) Protoplasm of Dendrobium candidum cryopreserved by vitrification. J Wenzhou Teach Coll (Nat Sci) 21:40–41 (in Chinese with English abstract)Google Scholar
  9. Chen Y, Wang JH, Huang CL (2001) Germplasm cryopreservation of Dendrobium candidum by vitrification. J Zhejinag Uni (Agric Life Sci) 27:436–438 (in Chinese with English abstract)Google Scholar
  10. Ching LP, Antony JJJ, Poobathy R, Subramaniam S (2012) Encapsulation–vitrification of Dendrobium sonia-28 supported by histology. Plant Omics J 5:345–350Google Scholar
  11. Das MC, Kumaria S, Tandon P (2008) In vitro propagation and conservation of Dendrobium lituiflorum Lindl. through protocorm-like bodies. J Plant Biochem Biotechnol 17:177–180CrossRefGoogle Scholar
  12. Devi PU, Selvi S, Devipriya D, Murugan S, Suja S (2009) Antitumor and antimicrobial activities and inhibition of in vitro lipid peroxidation of Dendrobium nobile. Afr J Biotechnol 8:2289–2293Google Scholar
  13. Ding G, Zhang D, Ding X, Zhou Q, Zhang W, Li X (2008) Genetic variation and conservation of the endangered Chinese endemic herb Dendrobium officinale based on SRAP analysis. Plant Syst Evol 276:149–156CrossRefGoogle Scholar
  14. Engelmann F (2011) Use of biotechnology for conservation of plant biodiversity. In Vitro Cell Dev Biol Plant 47:5–16CrossRefGoogle Scholar
  15. Engelmann F, Gonzalez-Arnao MT, Wu Y, Escobar R (2008) The development of encapsulation dehydration. In: Reed B (ed) Plant cryopreservation—a practical guide. Springer Science + Business Media LLC, New York, pp 59–76CrossRefGoogle Scholar
  16. Galdiano RF Jr, Lemos EGM, Faria RT, Vendrame WA (2012) Cryopreservation of Dendrobium hybrid seeds and protocorms as affected by phloroglucinol and Supercool X1000. Sci Hortic 148:154–160CrossRefGoogle Scholar
  17. Galdiano RF Jr, Lemos EGM, Faria RT, Vendrame WA (2014) Seedling development and evaluation of genetic stability of cryopreserved Dendrobium hybrid mature seeds. Appl Biochem Biotechnol 172:2521–2529PubMedCrossRefGoogle Scholar
  18. Harding K (2004) Genetic integrity of cryopreserved plant cells: a review. Cryo lett 25:3–22Google Scholar
  19. Hazubska-Przbyl T, Chmielarz P, Michalak M, Bojarczuk K (2010) Cryopreservation of embryogenic tissues of Picea omorika (Serbian spruce). Plant Cell Tissue Organ Cult 102:35–44CrossRefGoogle Scholar
  20. He MG, Wang RX, Song XQ, Song SQ, Zhang RL (2010) Study on cryopreservation of Dendrobium chrysanthum (Orchidaceae) seeds. Acta Bot Yunnanica 32:334–338 (in Chinese with English abstract)Google Scholar
  21. Hirano T, Godo T, Mii M, Ishikawa K (2005a) Cryopreservation of immature seeds of Bletilla striata by vitrification. Plant Cell Rep 23:534–539PubMedCrossRefGoogle Scholar
  22. Hirano T, Ishikawa K, Mii M (2005b) Cryopreservation of immature seeds of Ponerorchis graminifolia by vitrification. Cryo Lett 26:139–146Google Scholar
  23. Hirano T, Ishikawa K, Mii M (2006) Advances in orchid cryopreservation. In: Teixeira da Silva JA (ed) Floriculture, ornamental and plant biotechnology: advances and topical issues, vol II Global Science Books, Ltd., Isleworth, UK, pp 410–414Google Scholar
  24. Hossain MM, Kant R, Van PT, Winarto B, Zeng SJ, Teixeira da Silva JA (2013) The application of biotechnology to orchids. Crit Rev Plant Sci 32:69–139CrossRefGoogle Scholar
  25. Hou B, Tian M, Luo J, Ji Y, Xue Q, Ding X (2012) Genetic diversity assessment and ex situ conservation strategy of the endangered Dendrobium officinale (Orchidaceae) using new trinucleotide microsatellite markers. Plant Syst Evol 298:1483–1491CrossRefGoogle Scholar
  26. Huehne PS, Bhinja K (2012) Application of cryoprotectants to improve low temperature storage survival of orchid seeds. Sci Hortic 135:186–193CrossRefGoogle Scholar
  27. Kulus D, Zalewska M (2014) Cryopreservation as a tool used in long-term storage of ornamental species—a review. Sci Hortic 168:88–107CrossRefGoogle Scholar
  28. Lin WQ, Bian HW, Wang JH, Zhu MY (2004) Analysis of dehydrins in cryopreservation of protocorm-like-bodies of Dendrobium candidum by the air-drying method. Acta Hor Sin 31:64–68 (in Chinese with English abstract)Google Scholar
  29. Liu XD, Li XD, Wu YL, Shen XH (2013) Physiological and biochemical characteristic of Dendrobium wardianum protocorms during cryopreservation. J Northeast For Uni 41:79–84 (in Chinese with English abstract)Google Scholar
  30. Lurswijidjarus W, Thammasiri K (2004) Cryopreservation of shoot tips of Dendrobium Walter Oumae by encapsulation/dehydration. Sci Asia 30:293–299CrossRefGoogle Scholar
  31. Merritt DJ, Hay FR, Swarts ND, Sommerville KD, Dixon KW (2014) Ex situ conservation and cryopreservation of orchid germplasm. Int J Plant Sci 175:46–58CrossRefGoogle Scholar
  32. Mohanty P, Das J (2013) Synthetic seed technology for short term conservation of medicinal orchid Dendrobium densiflorum Lindl. Ex Wall and assessment of genetic fidelity of regenerants. Plant Growth Reg 70(297–303):305 (erratum; retracted)CrossRefGoogle Scholar
  33. Mohanty P, Das MC, Kumaria S, Tandon P (2012) High-efficiency cryopreservation of the medicinal orchid Dendrobium nobile Lindl. Plant Cell Tissue Organ Cult 109:297–305CrossRefGoogle Scholar
  34. Mohanty P, Das MC, Kumaria S, Tandon P (2013a) Cryopreservation of pharmaceutically important orchid Dendrobium chrysanthum Wall. ex Lindl. using vitrification based method. Acta Physiol Plant 35:1373–1379Google Scholar
  35. Mohanty P, Nongkling P, Das MC, Kumaria S, Tandon P (2013b) Short-term storage of alginate-encapsulated protocorm-like bodies of Dendrobium nobile Lindl.: an endangered medicinal orchid from North-east India. 3. Biotech 3:235–239Google Scholar
  36. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:493–497CrossRefGoogle Scholar
  37. Negash A, Krens F, Schaart J, Visser B (2001) In vitro conservation of enset under slow-growth conditions. Plant Cell Tissue Organ Cult 66:107–111CrossRefGoogle Scholar
  38. Ng TB, Liu JY, Wong JH, Ye XJ, Sze SCW, Tong Y, Zhang KYB (2012) Review of research on Dendrobium, a prized folk medicine. Appl Microbiol Biotechnol 93:1795–1803PubMedCrossRefGoogle Scholar
  39. Nikishina TV, Popova EV, Vakhrameeva MG, Varlygina TI, Kolomeitseva GL, Burov AV, Popovich EA, Shirokov AI, Shumilov VYu, Popov AS (2007) Cryopreservation of seeds and protocorms of rare temperate orchids. Russ J Plant Physiol 54:121–127Google Scholar
  40. Park SY, Murthy HN, Chakrabarthy D, Paek KY (2009) Detection of epigenetic variation in tissue-culture-derived plants of Doritaenopsis by methylation-sensitive amplification polymorphism (MSAP) analysis. In Vitro Cell Dev Biol Plant 45:104–108CrossRefGoogle Scholar
  41. Poobathy R, Izwa N, Julkifle AL, Subramaniam S (2013a) Cryopreservation of Dendrobium sonia-28 using an alternative method of PVS2 droplet freezing. Emir J Food Agric 25:531–538Google Scholar
  42. Poobathy R, Sinniah UR, Mahmood M, Subramaniam S (2013b) Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28. Turk J Bot 37:940–949CrossRefGoogle Scholar
  43. Poobathy R, Sinniah UR, Xavier R, Subramaniam S (2013c) Catalase and superoxide dismutase activities and the total protein content of protocorm-like bodies of Dendrobium Sonia-28 subjected to vitrification. Appl Biochem Biotechnol 170:1066–1079PubMedCrossRefGoogle Scholar
  44. Pradhan S, Paudel YP, Pant B (2013) Efficient regeneration of plants from shoot tip explants of Dendrobium densiflorum LindL., a medicinal orchid. Afr J Biotechnol 12:1378–1383Google Scholar
  45. Pridgeon A, Morrison A (2006) The illustrated encyclopedia of orchids–over 1100 species illustrated and identified. Timber Press, Ltd., Portland pp 87–103Google Scholar
  46. Pritchard HW (1984) Liquid nitrogen preservation of terrestrial and epiphytic orchid seed. Cryo Lett 5:295–300Google Scholar
  47. Pritchard HW (1985) Determination of orchid seed viability using fluorescein diacetate. Plant Cell Environ 8:727–730Google Scholar
  48. Pritchard HW, Poyner ALC, Seaton PT (1999) Interspecific variation in orchid seed longevity in relation to ultra-dry storage and cryopreservation. Lindleyana 14:92–101Google Scholar
  49. Puchooa D (2004) Comparison of different culture media for the in vitro culture of Dendrobium (Orchidaceae). Int J Agric Biol 6:884–888Google Scholar
  50. Sakai A, Engelmann F (2007) Vitrification, encapsulation–vitrification and droplet-vitrification: a review. Cryo Lett 28:151–172Google Scholar
  51. Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9:30–33PubMedCrossRefGoogle Scholar
  52. Sakai A, Kobayashi S, Oiyama I (1991) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) cooled to −196 °C. J Plant Physiol 137:465–470CrossRefGoogle Scholar
  53. Sakai A, Hiraii D, Niino T (2008) Development of PVS-based vitrification and encapsulation–vitrification protocols. In: Reed B (ed) Plant cryopreservation—a practical guide. Springer Science Business Media, LLC, New York, pp 33–58CrossRefGoogle Scholar
  54. Shefferson RP, Weiss M, Kull T, Taylor DL (2005) High specificity generally characterizes mycorrhizal association in rare lady’s slipper orchids, genus Cypripedium. Mol Ecol 14:613–626PubMedCrossRefGoogle Scholar
  55. Shi YZ, Pan RH, Wang XJ, Ye QS, Guo LR (1999) In vitro conservation of germplasm at room temperature in Dendrobium officinale. J South China Normal Uni (Nat Sci) 4:73–77 (in Chinese with English abstract)Google Scholar
  56. Shi YZ, Pan RH, Wang XJ, Ye QS, Guo LR (2000) In vitro conservation of Dendrobium officinale at low temperature. Chin J Appl Environ Biol 6:326–330 (in Chinese with English abstract)Google Scholar
  57. Singh F (1981) Differential staining of orchid seeds for viability testing. Am Orchid Soc Bull 50:416–418Google Scholar
  58. Subramaniam S, Sinniah UR, Khoddamzadeh AA, Periasamy S, James JJ (2011) Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation–dehydration technique. Afr J Biotechnol 10:3902–3907Google Scholar
  59. Surenciski MR, Dematteis M, Flachsland EA (2007) Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae). Ann Bot Fennici 44:287–292Google Scholar
  60. Swarts ND, Dixon KW (2009) Terrestrial orchid conservation in the age of extinction. Ann Bot 104:543–556PubMedCentralPubMedCrossRefGoogle Scholar
  61. Teixeira da Silva JA (2012) Is BA (6-benzyladenine) BAP (6-benzylaminopurine)? Asian Australasian J Plant Sci Biotech 6(Special Issue 1):121–124Google Scholar
  62. Teixeira da Silva JA (2013a) Orchids: advances in tissue culture, genetics, phytochemistry and transgenic biotechnology. Floric Ornam Biotech 7:1–52Google Scholar
  63. Teixeira da Silva JA (2013b) Cryopreservation of hybrid Cymbidium protocorm-like bodies by encapsulation–dehydration and vitrification. In Vitro Cell Dev Biol Plant 49:690–698CrossRefGoogle Scholar
  64. Teixeira da Silva JA, Tanaka M (2006) Embryogenic callus, PLB and TCL paths to regeneration in hybrid Cymbidium (Orchidaceae). J Plant Growth Reg 25:203–210CrossRefGoogle Scholar
  65. Teixeira da Silva JA, Dobránszki J, Ross S (2013) Phloroglucinol in plant tissue culture. In Vitro Cell Dev Biol Plant 49:1–16CrossRefGoogle Scholar
  66. Tiau TK, Rathinam X, Keng CL, Subramaniam S (2009) An assessment of early factors influencing the PVS2 vitrification method using protocorm-like bodies of Dendrobium Sonia 28. Am Eurasian J Sustain Agric 3:280–289Google Scholar
  67. US Department of Agriculture (2011) Floriculture crops 2010 summary. National Agricultural Statistics Service. Washington, DC. Online: http://usda.mannlib.cornell.edu/usda/nass/FlorCrop//2010s/2011/FlorCrop-04-21-2011_revision.pdf. Accessed 14 May 2014
  68. US Department of Agriculture (2012) Floriculture crops 2011 summary. National Agricultural Statistics Service. Washington, DC. Online: http://usda.mannlib.cornell.edu/usda/nass/FlorCrop//2010s/2012/FlorCrop-05-31-2012.pdf. Accessed 14 May 2014
  69. US Department of Agriculture (2013) Floriculture crops 2012 summary. National Agricultural Statistics Service. Washington, DC. Online: http://usda.mannlib.cornell.edu/usda/current/FlorCrop/FlorCrop-04-25-2013.pdf. Accessed 14 May 2014
  70. Vacin E, Went FW (1949) Some pH changes in nutrient solutions. Bot Gaz 110:605–613CrossRefGoogle Scholar
  71. Vendrame WA, Faria RT (2011) Phloroglucinol enhances recovery and survival of cryopreserved Dendrobium nobile protocorms. Sci Hortic 128:131–135CrossRefGoogle Scholar
  72. Vendrame WA, Carvalho VS, Dias JMM (2007) In vitro germination and seedling development of cryopreserved Dendrobium hybrid mature seeds. Sci Hortic 114:188–193CrossRefGoogle Scholar
  73. Vendrame WA, Carvalho VS, Dias JMM, Maguire I (2008) Pollination of Dendrobium hybrids using cryopreserved pollen. HortSci 43:264–267Google Scholar
  74. Verleysen H, Samyn G, Van Bockstaele E, Debergh P (2004) Evaluation of analytical techniques to predict viability after cryopreservation. Plant Cell Tissue Organ Cult 77:11–21CrossRefGoogle Scholar
  75. Wang JH, Ge J-G, Liu F, Bian H-W, Huang CN (1998) Cryopreservation of seeds and protocorms of Dendrobium candidum. Cryo Lett 19:123–128Google Scholar
  76. Wang JH, Zhang YQ, Liu F, Huang CL, Ge JG (1999) Cryopreservation of seeds, protocorm and protocorm-like bodies of Dendrobium candidum. Acta Hort Sin 26:59–61 (in Chinese with English abstract)Google Scholar
  77. Wang JH, Luo JP, Zha XQ, Feng BJ (2010) Comparison of antitumor activities of different polysaccharide fractions from the stems of Dendrobium nobile Lindl. Carbohydr Polym 79:114–118CrossRefGoogle Scholar
  78. Wu YL, Shen XH (2011) Cryopreservation of Dendrobium wardianum Warner protocorms by vitrification. Chin J Cell Bio 33:279–287 (in Chinese with English abstract)Google Scholar
  79. Xu H, Wang ZT, Ding XY, Zhou KY, Xu LS (2006) Differentiation of Dendrobium species used as “Huangcao Shihu” by rDNA ITS sequence analysis. Planta Med 72:89–92PubMedCrossRefGoogle Scholar
  80. Yam TW, Chua J, Tay F, Ang P (2010) Conservation of the native orchids through seedling culture and reintroduction—a Singapore experience. Bot Rev 76:263–274CrossRefGoogle Scholar
  81. Yin MH, Hong SR (2009) Cryopreservation of Dendrobium candidum wall. ex Lindl. protocorm-like bodies by encapsulation vitrification. Plant Cell Tissue Organ Cult 98:179–185CrossRefGoogle Scholar
  82. Zainuddin M, Julkifle AL, Pobathy R, Sinniah UR, Antony JJJK, Pavallekoodi, Subramaniam S (2011) Preliminary analysis of cryopreservation of Dendrobium Bobby Messina orchid using an encapsulation–dehydration technique with Evans blue. Afr J Biotechnol 10:11870–11878Google Scholar
  83. Zhang ZG, Liu H, Xia ZJ, Wang JH (1997) Cryopreservation of seeds of Dendrobium candidum. J Anhui Coll 6:40–42 (in Chinese with English abstract)Google Scholar
  84. Zhang M, Wei XY, Huang HR (2001) A study on the solid encapsulating system of the artificial seed of Dendrobium candidum. Acta Hort Sin 28:435–439 (in Chinese with English abstract)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.IkenobeJapan
  2. 2.Key Laboratory of South China Agricultural Plant Genetics and Breeding, South China Botanical GardenChinese Academy of SciencesBeijingChina
  3. 3.Department of TechnologyUniversidade Estadual PaulistaJaboticabalBrazil
  4. 4.Research Institute of Nyíregyháza, Research Institutes and Study Farm, Centre for Agricultural and Applied Economic SciencesUniversity of DebrecenNyíregyházaHungary
  5. 5.Department of Rural DevelopmentCentro de Ciências Agrárias, UFSCarArarasBrazil
  6. 6.Tropical Research and Education CenterUniversity of FloridaHomesteadUSA

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