Plant Cell Reports

, Volume 32, Issue 12, pp 1827–1841

Wood chemistry analysis and expression profiling of a poplar clone expressing a tyrosine-rich peptide

  • Yi Xu
  • Chin-Fu Chen
  • Tina P. Thomas
  • Parastoo Azadi
  • Brett Diehl
  • Chung-Jui Tsai
  • Nicole Brown
  • John E. Carlson
  • Ming Tien
  • Haiying Liang
Original Paper

DOI: 10.1007/s00299-013-1496-0

Cite this article as:
Xu, Y., Chen, CF., Thomas, T.P. et al. Plant Cell Rep (2013) 32: 1827. doi:10.1007/s00299-013-1496-0

Abstract

Key message

Our study has identified pathways and gene candidates that may be associated with the greater flexibility and digestibility of the poplar cell walls.

Abstract

With the goal of facilitating lignin removal during the utilization of woody biomass as a biofuel feedstock, we previously transformed a hybrid poplar clone with a partial cDNA sequence encoding a tyrosine- and hydroxyproline-rich glycoprotein from parsley. A number of the transgenic lines released more polysaccharides following protease digestion and were more flexible than wild-type plants, but otherwise normal in phenotype. Here, we report that overexpression of the tyrosine-rich peptide encoding sequence in these transgenic poplar plants did not significantly alter total lignin quantity or quality (S/G lignin ratio), five- and six-carbon sugar contents, growth rate, or susceptibility to a major poplar fungal pathogen, Septoria musiva. Whole-genome microarray analysis revealed a total of 411 differentially expressed transcripts in transgenic lines, all with decreased transcript abundance relative to wild-type plants. Their corresponding genes were overrepresented in functional categories such as secondary metabolism, amino acid metabolism, and energy metabolism. Transcript abundance was decreased primarily for five types of genes encoding proteins involved in cell-wall organization and in lignin biosynthesis. The expression of a subset of 19 of the differentially regulated genes by qRT-PCR validated the microarray results. Our study has identified pathways and gene candidates that may be the underlying cause for the enhanced flexibility and digestibility of the stems of poplar plants expressing the TYR transgene.

Keywords

Cell-wall composition Differential gene expression Poplar Pyrolysis molecular beam mass spectrometry (pyMBMS) Tyrosine-rich peptide 

Supplementary material

299_2013_1496_MOESM1_ESM.doc (141 kb)
Supplementary material 1 (DOC 141 kb)
299_2013_1496_MOESM2_ESM.xlsx (386 kb)
Supplementary material 2 (XLSX 386 kb)
299_2013_1496_MOESM3_ESM.xlsx (14 kb)
Supplementary material 3 (XLSX 13 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Yi Xu
    • 1
  • Chin-Fu Chen
    • 2
  • Tina P. Thomas
    • 3
  • Parastoo Azadi
    • 4
  • Brett Diehl
    • 5
  • Chung-Jui Tsai
    • 6
  • Nicole Brown
    • 5
  • John E. Carlson
    • 7
    • 8
  • Ming Tien
    • 9
  • Haiying Liang
    • 1
  1. 1.Department of Genetics and BiochemistryClemson UniversityClemsonUSA
  2. 2.Center for Molecular StudiesGreenwood Genetic CenterGreenwoodUSA
  3. 3.Bioenergy Systems Research InstituteUniversity of GeorgiaAthensUSA
  4. 4.Complex Carbohydrate Research CenterUniversity of GeorgiaAthensUSA
  5. 5.Department of Agricultural and Biological EngineeringPennsylvania State UniversityUniversity ParkUSA
  6. 6.Warnell School of Forestry and Natural Resources and Department of GeneticsUniversity of GeorgiaAthensUSA
  7. 7.The Department of Ecosystem Science and Management and The Department of Plant SciencePennsylvania State UniversityUniversity ParkUSA
  8. 8.Department of Bioenergy Science and Technology (World Class University)Chonnam National University, Buk-GuGwangjuKorea
  9. 9.Department of Biochemistry and Molecular BiologyPennsylvania State UniversityUniversity ParkUSA

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