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Plant Cell Reports

, Volume 32, Issue 10, pp 1557–1574 | Cite as

Agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane

  • Subramanian Mayavan
  • Kondeti Subramanyam
  • Muthukrishnan Arun
  • Manoharan Rajesh
  • Gnanajothi Kapil Dev
  • Ganeshan Sivanandhan
  • Balusamy Jaganath
  • Markandan Manickavasagam
  • Natesan Selvaraj
  • Andy GanapathiEmail author
Original Paper

Abstract

Key message

An efficient, reproducible and genotype-independent in planta transformation has been standardized for sugarcane using seed as explant.

Abstract

Transgenic sugarcane production through Agrobacterium infection followed by in vitro regeneration is a time-consuming process and highly genotype dependent. To obtain more number of transformed sugarcane plants in a relatively short duration, sugarcane seeds were infected with Agrobacterium tumefaciens EHA 105 harboring pCAMBIA 1304-bar and transformed plants were successfully established without undergoing in vitro regeneration. Various factors affecting sugarcane seed transformation were optimized, including pre-culture duration, acetosyringone concentration, surfactants, co-cultivation, sonication and vacuum infiltration duration. The transformed sugarcane plants were selected against BASTA® and screened by GUS and GFP visual assay, PCR and Southern hybridization. Among the different combinations and concentrations tested, when 12-h pre-cultured seeds were sonicated for 10 min and 3 min vacuum infiltered in 100 µM acetosyringone and 0.1 % Silwett L-77 containing Agrobacterium suspension and co-cultivated for 72-h showed highest transformation efficiency. The amenability of the standardized protocol was tested on five genotypes. It was found that all the tested genotypes responded favorably, though CoC671 proved to be the best responding cultivar with 45.4 % transformation efficiency. The developed protocol is cost-effective, efficient and genotype independent without involvement of any tissue culture procedure and can generate a relatively large number of transgenic plants in approximately 2 months.

Keywords

Agrobacterium tumefaciens EHA105 BASTA® In planta transformation Seed fluff Southern blot hybridization Sugarcane 

Abbreviations

MS

Murashige and Skoog medium

GA3

Gibberellic acid

hpt II

Hygromycin phosphotransferase

npt II

Neomycin phosphotransferase

gfpgus

Green fluorescent protein-β-glucuronidase fusion gene

CaMV 35S

Cauliflower mosaic virus 35S promoter

Notes

Acknowledgments

The authors are grateful to the University Grants Commission (UGC), Government of India, for the financial support [No.F. 2-3/2011 (Policy/SR)] to carry out the present work. The corresponding author is thankful to University Grants Commission (UGC), Govt. of India, for providing fellowship under UGC-BSR scheme. Subramanyam and Sivanadhan are thankful to the Council of Scientific and Industrial Research (CSIR), Govt. Of India, for the award of Senior Research Fellowship (SRF) for their doctoral research.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Subramanian Mayavan
    • 1
    • 2
  • Kondeti Subramanyam
    • 1
  • Muthukrishnan Arun
    • 1
    • 3
  • Manoharan Rajesh
    • 1
  • Gnanajothi Kapil Dev
    • 1
  • Ganeshan Sivanandhan
    • 1
  • Balusamy Jaganath
    • 1
  • Markandan Manickavasagam
    • 1
  • Natesan Selvaraj
    • 4
  • Andy Ganapathi
    • 1
    Email author
  1. 1.Department of Biotechnology and Genetic Engineering, School of BiotechnologyBharathidasan UniversityTiruchirappalliIndia
  2. 2.Synthetic Biology and Biofuel GroupInternational Center for Genetic Engineering and Biotechnology (ICGEB)New DelhiIndia
  3. 3.Department of Genetic Engineering, School of BioengineeringSRM UniversityKancheepuram DistrictIndia
  4. 4.Periyar E.V.R College (Autonomous)TiruchirappalliIndia

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