Plant Cell Reports

, Volume 32, Issue 9, pp 1455–1463 | Cite as

Fine mapping of BH1, a gene controlling lemma and palea development in rice

  • Xiangjin Wei
  • Xuanwen Zhang
  • Gaoneng Shao
  • Jiwai He
  • Guiai Jiao
  • Lihong Xie
  • Zhonghua Sheng
  • Shaoqing TangEmail author
  • Peisong HuEmail author
Original Paper


Key message

A new rice floral organ mutant bh1 , had a negative effect on grain yield. BH1 was fine mapped to 87.5 kb on chr2. A 55 kb chromosome segment was deleted in bh1.


The cereal spikelet is enclosed by the lemma and palea. The lemma and palea of the floral mutant designated bh1, a selection from a T-DNA library generated from the rice cultivar Asominori, takes on an abnormal curve-shaped appearance only late in floral development, finally forming a beak-shaped hull. The mutation had a negative effect on thousand grain weight, seed set rate and germination rate. Genetic analysis indicated that the mutant phenotype was determined by a single recessive gene. Through map-based approach, BH1 gene was finally located to a ~87.5-kbp region on the long arm of chromosome 2. An analysis of the gene content of this region indicated that the mutation involves the loss of a 55-kbp stretch, harboring four open reading frames. Transcription profiling based on qRT-PCR revealed that the genes OsMADS1, OsMADS14, OsMADS15, OsMADS18, REP1, CFO1, and DL, all of which are also involved in lemma and palea development and identity specification, were down-regulated in the bh1 mutant. BH1 is therefore an important floral organ development gene.


Gene mapping Beak-shaped hull Floral organ Chromosome segment deletion Rice 



We acknowledge the financial support given by the Natural Science Foundation of China (31201195), the Zhejiang province Science and Technology Project (2010C32061), the Chinese government “863” Program (2011AA10A101, 2012AA101101), the National S and T Major Project (2011ZX08001-001, 2011ZX08001-002 and 2011ZX08001-006), and the Central level, non-profit, scientific research institutes basic R and D operations Special Fund (2012RG002-1).


  1. Ciaffi M, Paolacci AR, Tanzarella OA, Porceddu E (2011) Molecular aspects of flower development in grasses. Sex Plant Reprod 24(4):247–282PubMedCrossRefGoogle Scholar
  2. Coen ES, Meyerowitz EM (1991) The war of the whorls: genetic interactions controlling flower development. Nature 353:31–37PubMedCrossRefGoogle Scholar
  3. Ditta G, Pinyopich A, Robles P, Pelaz S, Yanofsky MF (2004) The SEP4 gene of Arabidopsis thaliana functions in floral organ and meristem identity. Curr Biol 14:1935–1940PubMedCrossRefGoogle Scholar
  4. Dreni L, Jacchia S, Fornara F, Fornari M, Ouwerkerk PB, An G, Colombo L, Kater MM (2007) The D-lineage MADS-box gene OsMADS13 controls ovule identity in rice. Plant J 52:690–699PubMedCrossRefGoogle Scholar
  5. Fornara F, Parenicová L, Falasca G, Pelucchi N, Masiero S, Ciannamea S, Lopez-Dee Z, Altamura MM, Colombo L, Kater MM (2004) Functional characterization of OsMADS18, a member of the AP1/SQUA subfamily of MADS-box genes. Plant Physiol 135:2207–2219PubMedCrossRefGoogle Scholar
  6. Immink RG, Kaufmann K, Angenent GC (2010) The ‘ABC’ of MADS domain protein behaviour and interactions. Semin Cell Dev Biol 21:87–93PubMedCrossRefGoogle Scholar
  7. Itoh JI, Nonomura KI, Ikeda K, Yamaki S, Inukai Y, Yamagishi H, Kitano H, Nagato Y (2005) Rice plant development: from zygote to spikelet. Plant Cell Physiol 46:23–47PubMedCrossRefGoogle Scholar
  8. Jeon JS, Jang S, Lee S, Nam J, Kim C, Lee SH, Chung YY, Kim SR, Lee YH, Cho YG, An G (2000) Leaf hull sterile1 is a homeotic mutation in a rice MADS-box gene affecting rice flower development. Plant Cell 12:871–884PubMedGoogle Scholar
  9. Kang HG, Jeon JS, Lee S, An G (1998) Identification of class B and class C floral organ identity genes from rice plants. Plant Mol Biol 38:1021–1029PubMedCrossRefGoogle Scholar
  10. Kater MM, Dreni L, Colombo L (2006) Functional conservation of MADS-box factors controlling floral organ identity in rice and Arabidopsis. J Exp Bot 57:3433–3444PubMedCrossRefGoogle Scholar
  11. Kyozuka J, Shimamoto K (2002) Ectopic expression of OsMADS3, a rice ortholog of AGAMOUS, caused a homeotic transformation of lodicules to stamens in transgenic rice plants. Plant Cell Physiol 43:130–135PubMedCrossRefGoogle Scholar
  12. Kyozuka J, Kobayashi T, Morita M, Shimamoto K (2000) Spatially and temporally regulated expression of rice MADS-box genes with similarity to Arabidopsis class A, B and C genes. Plant Cell Physiol 41:710–718PubMedCrossRefGoogle Scholar
  13. Li A, Zhang Y, Wu X, Tang W, Wu R, Dai Z, Liu G, Zhang H, Wu C, Chen G, Pan X (2008) DH1, a LOB domain-like protein required for glume formation in rice. Plant Mol Biol 66:491–502PubMedCrossRefGoogle Scholar
  14. Li H, Liang W, Jia R, Yin C, Zong J, Kong H, Zhang D (2010) The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice. Cell Res 20:299–313PubMedCrossRefGoogle Scholar
  15. Li H, Liang W, Hu Y, Zhu L, Yin C, Xu J, Dreni L, Kater M, Zhang D (2011) Rice MADS6 interacts with the floral homeotic genes SUPERWOMAN1, MADS3, MADS58, MADS13, and DROOPING LEAF in specifying floral organ identities and meristem fate. Plant Cell 23:2536–2552PubMedCrossRefGoogle Scholar
  16. Li XJ, Sun LJ, Tan LB, Liu FX, Zhu ZF, Fu YC, Sun XY, Sun XW, Xie DX, Sun CQ (2012) TH1, a DUF640 domain-like gene controls lemma and palea development in rice. Plant Mol Biol 78:351–359PubMedCrossRefGoogle Scholar
  17. Lohmann JU, Hong RL, Hobe M, Busch MA, Parcy F, Simon R, Weigel D (2001) A molecular link between stem cell regulation and floral patterning in Arabidopsis. Cell 105:793–803PubMedCrossRefGoogle Scholar
  18. Lopez-Dee ZP, Wittich P, Enrico Pè M, Rigola D, Del Buono I, Gorla MS, Kater MM, Colombo L (1999) OsMADS13, a novel rice MADS-box gene expressed during ovule development. Dev Genet 25:237–244PubMedCrossRefGoogle Scholar
  19. Luo Q, Zhou KD, Zhao XF, Zeng QC, Xia HA, Zhai WX, Xu JC, Wu XJ, Yang HS, Zhu LH (2005) Identification and fine mapping of a mutant gene for palealess spikelet in rice. Planta 221:222–230PubMedCrossRefGoogle Scholar
  20. Ma XD, Cheng ZJ, Wu FQ, Jin MN, Zhang LG, Zhou F, Wang JL, Zhou KN, Ma J, Lin QB, Lei CL, Wan JM (2012) BEAK LIKE SPIKELET1 is required for lateral development of lemma and palea in rice. Plant Mol Biol Rep 31(1):98–108CrossRefGoogle Scholar
  21. Moon YH, Kang HG, Jung JY, Jeon JS, Sung SK, An G (1999) Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol 120:1193–1204PubMedCrossRefGoogle Scholar
  22. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325PubMedCrossRefGoogle Scholar
  23. Nagasawa N, Miyoshi M, Sano Y, Satoh H, Hirano H, Sakai H, Nagato Y (2003) SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice. Development 130:705–718PubMedCrossRefGoogle Scholar
  24. Ohmori S, Kimizu M, Sugita M, Miyao A, Hirochika H, Uchida E, Nagato Y, Yoshida H (2009) MOSAIC FLORAL ORGANS1, an AGL6-like MADS-box gene, regulates floral organ identity and meristem fate in rice. Plant Cell 21:3008–3025PubMedCrossRefGoogle Scholar
  25. Pelucchi N, Fornara F, Favalli C, Masiero S, Lago C, Enrico Pè M, Colombo L, Kater MM (2002) Comparative analysis of rice MADS-box genes expressed during flower development. Sex Plant Reprod 15:113–122CrossRefGoogle Scholar
  26. Prasad K, Vijayraghavan U (2003) Double-stranded RNA interference of a rice PI/GLO paralog OsMADS2, uncovers its second-whorl-specific function in floral organ patterning. Genetics 165:2301–2305PubMedGoogle Scholar
  27. Prasad K, Parameswaran S, Vijayraghavan U (2005) OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs. Plant J 43:915–928PubMedCrossRefGoogle Scholar
  28. Ren DY, Li YF, Wang Z, Xu FF, Guo S, Zhao FM, Sang XC, Ling YH, He GH (2012) Identification and gene mapping of a multi-floret spikelet 1 (mfs1) mutant associated with spikelet development in rice. J Integr Agric 11:1574–1579CrossRefGoogle Scholar
  29. Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissues. Plant Mol Biol 5:69–76CrossRefGoogle Scholar
  30. Sang X, Li Y, Luo Z, Ren D, Fang L, Wang N, Zhao F, Ling Y, Yang Z, Liu Y, He G (2012) CHIMERIC FLORAL ORGANS1, encoding a monocot-specific MADS-box protein, regulates floral organ identity in rice. Plant Physiol 160:788–807PubMedCrossRefGoogle Scholar
  31. Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc 3:1101–1108PubMedCrossRefGoogle Scholar
  32. Theissen G, Saedler H (2001) Plant biology: floral quartets. Nature 409:469–471PubMedCrossRefGoogle Scholar
  33. Wang N, Sang XC, Li YF, Yang ZL, Zhao FM, Ling YH, Zhang ZS, He GH (2010) Identification and gene mapping of a novel mutant supernumerary lodicules (snl) in rice. J Integr Plant Biol 52:265–272PubMedCrossRefGoogle Scholar
  34. Weigel D, Meyerowitz EM (1994) The ABCs of floral homeotic genes. Cell 78:203–209PubMedCrossRefGoogle Scholar
  35. Yamaguchi T, Hirano HY (2006) Function and diversification of MADS-box genes in rice. Sci World J 6:1923–1932CrossRefGoogle Scholar
  36. Yamaguchi T, Nagasawa N, Kawasaki S, Matsuoka M, Nagato Y, Hirano HY (2004) The YABBY gene DROOPING LEAF regulates carpel specification and midrib development in Oryza sativa. Plant Cell 16:500–509PubMedCrossRefGoogle Scholar
  37. Yamaguchi T, Lee DY, Miyao A, Hirochika H, An G, Hirano HY (2006) Functional diversification of the two C-class genes OsMADS3 and OsMADS58 in Oryza sativa. Plant Cell 18:15–18PubMedCrossRefGoogle Scholar
  38. Yoshida H, Nagato Y (2011) Flower development in rice. J Exp Bot 62(14):4719–4730PubMedCrossRefGoogle Scholar
  39. Yuan Z, Gao S, Xue DW, Luo D, Li LT, Ding SY, Yao X, Wilson ZA, Qian Q, Zhang DB (2009) RETARDED PALEA1 controls palea development and floral zygomorphy in rice. Plant Physiol 149:235–244PubMedCrossRefGoogle Scholar
  40. Zhang Q, Shen BZ, Dai XK, Mei MH, Saghai MA, Li ZB (1994) Using bulked extremes and recessive classes to map genes for photoperiod-sensitive genic male sterility in rice. Proc Natl Acad Sci 91:8675–8679PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Xiangjin Wei
    • 1
  • Xuanwen Zhang
    • 1
  • Gaoneng Shao
    • 1
  • Jiwai He
    • 1
  • Guiai Jiao
    • 1
  • Lihong Xie
    • 1
  • Zhonghua Sheng
    • 1
  • Shaoqing Tang
    • 1
    Email author
  • Peisong Hu
    • 1
    Email author
  1. 1.State Key Laboratory of Rice Biology, Key Laboratory of Rice Biology and Breeding of Ministry of AgricultureChina National Rice Research InstituteHangzhouChina

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