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Polar protein transport between apical and basal cells during tobacco early embryogenesis

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Abstract

Key message

We found that protein trafficking between apical and basal cell can be unidirectional, which reveals the different roles of the two cells in the cell-to-cell communication between them during early embryogenesis.

Abstract

In most angiosperm species, asymmetric zygote division results in an apical cell and a basal cell that have distinct cell fates. Much has been speculated about possible communication between these cell types in relation to their cell fate determination. Here, we report on the use of photoactivatable green fluorescent protein (PA-GFP) in tobacco to trace intercellular communication between apical and basal cells during early embryogenesis. We found that PA-GFP was transported between apical and basal cells of a two-celled proembryo, and that protein trafficking was unidirectional toward the apical cell, highlighting different cell communication roles. Further ultrastructural analysis showed numerous plasmodesmata in the walls connecting the apical and basal cells, which may provide channels for protein trafficking. Our data show a possible unique method of cell-to-cell communication between apical and basal cells during early embryogenesis.

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Abbreviations

AXR6:

AUXIN RESPONSE 6

CLSM:

Confocal laser scanning microscope

cDNA:

Complementary deoxyribonucleic acid

GFP:

Green fluorescent protein

MAPKKK:

Mitogen-activated protein kinase kinase kinase

MP:

MONOPTEROS

NCAPs:

Non-cell autonomous proteins

ORF:

Open-reading frame

PA-GFP:

Photoactivatable green fluorescent protein

PD:

Plasmodesmata

PIN:

PINFORMED

RAM:

Root apical meristem

RNA:

Ribonucleic acid

sGFP:

Soluble green fluorescent protein

TMO7:

Target of MONOPTEROS 7

UV:

Ultraviolet

YDA:

YODA

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Acknowledgments

The project was supported by the “973” Program (2013CB126900), National Natural Science Fund of China (31170297; 31070284). We warmly thank Prof. Oparka from University of Edinburgh for his generous gift of T-Easy Vector containing PA-GFP.

Author information

Correspondence to Meng-xiang Sun.

Additional information

Communicated by M. Otegui.

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Supplementary material 3 (MPG 916 kb)

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Li, S., He, Y., Zhao, J. et al. Polar protein transport between apical and basal cells during tobacco early embryogenesis. Plant Cell Rep 32, 285–291 (2013). https://doi.org/10.1007/s00299-012-1362-5

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Keywords

  • Photoactivatable green fluorescent protein (PA-GFP)
  • Cell-to-cell communication
  • Plasmodesmata
  • Apical cell
  • Basal cell
  • Protein trafficking