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Plant Cell Reports

, Volume 31, Issue 10, pp 1829–1843 | Cite as

EgRBP42 encoding an hnRNP-like RNA-binding protein from Elaeis guineensis Jacq. is responsive to abiotic stresses

  • Wan-Chin Yeap
  • Tony Eng Keong Ooi
  • Parameswari Namasivayam
  • Harikrishna Kulaveerasingam
  • Chai-Ling Ho
Original Paper

Abstract

Contents RNA-binding proteins (RBPs) have been implicated as regulatory proteins involved in the post-transcriptional processes of gene expression in plants under various stress conditions. In this study, we report the cloning and characterization of a gene, designated as EgRBP42, encoding a member of the plant heterogeneous nuclear ribonucleoprotein (hnRNP)-like RBP family from oil palm (Elaeis guineensis Jacq.). EgRBP42 consists of two N-terminal RNA recognition motifs and a glycine-rich domain at the C-terminus. The upstream region of EgRBP42 has multiple light-responsive, stress-responsive regulatory elements and regulatory elements associated with flower development. Real-time RT-PCR analysis of EgRBP42 showed that EgRBP42 was expressed in oil palm tissues tested, including leaf, shoot apical meristem, root, female inflorescence, male inflorescence and mesocarp with the lowest transcript level in the roots. EgRBP42 protein interacted with transcripts associated with transcription, translation and stress responses using pull-down assay and electrophoretic mobility shift assay. The accumulation of EgRBP42 and its interacting transcripts were induced by abiotic stresses, including salinity, drought, submergence, cold and heat stresses in leaf discs. Collectively, the data suggested that EgRBP42 is a RBP, which responds to various abiotic stresses and could be advantageous for oil palm under stress conditions.

Key message EgRBP42 may be involved in the post-transcriptional regulation of stress-related genes important for plant stress response and adaptation.

Keywords

Abiotic stresses Elaeis guineensis Jacq. hnRNP-like proteins Oil palm RNA-binding proteins 

Notes

Acknowledgments

This study was financially supported by Sime Darby Plantation. The authors thank SDR Banting for the oil palm samples, Dr. Mohd Nazir Basiran, Mr. Wong Yick Ching and Mdm. Lee Fong Chin for helpful discussion. The authors also would like to thank Mdm. Alena Lee Sanusi and Dr. Mohd Sanusi Jangi for useful editorial comments on the manuscript.

Supplementary material

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Supplementary material 1 (DOCX 25 kb)
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Supplementary material 4 (XLSX 31 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Wan-Chin Yeap
    • 1
    • 2
  • Tony Eng Keong Ooi
    • 2
  • Parameswari Namasivayam
    • 1
    • 3
  • Harikrishna Kulaveerasingam
    • 2
  • Chai-Ling Ho
    • 1
    • 3
  1. 1.Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra MalaysiaSelangorMalaysia
  2. 2.Sime Darby Technology Centre Sdn Bhd, UPM-MTDC Technology Centre III, Universiti Putra MalaysiaSelangorMalaysia
  3. 3.Institute of Tropical Agriculture, Universiti Putra MalaysiaSelangorMalaysia

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