Exogenous thermospermine has an activity to induce a subset of the defense genes and restrict cucumber mosaic virus multiplication in Arabidopsis thaliana
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We previously proposed the defensive role of a signal pathway triggered by the polyamine spermine (Spm) in the reaction against avirulent viral pathogens in Nicotiana tabacum and Arabidopsis thaliana. In this study, we showed that thermospermine (T-Spm), an isomer of Spm, is also active in inducing the expression of the genes involved in the Spm-signal pathway at a similar dose as Spm. Furthermore, we found that T-Spm enhances the expression of a subset of pathogenesis-related genes whose expression is induced during cucumber mosaic virus (CMV)-triggered hypersensitive response. In consistent with the above observation, we further showed that exogenous T-Spm can repress CMV multiplication with same efficiency as Spm.
Polyamine thermospermine, an isomer of spermine, is able to induce a subset of hypersensitive response-related defense genes and can suppress cucumber mosaic virus multiplication in Arabidopsis thaliana.
KeywordsArabidopsis thaliana Cucumber mosaic virus Polyamine Signaling activity Spermine Thermospermine
This work was supported in part by Grant-in-Aids from the Japan Society for the Promotion of Science (JSPS) to TK (21380063) and YT (21780087), and by the grant from The Saito Gratitude Foundation (to GHMS) and by the research funding programme “LOEWE-Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz” of Hesse’s Ministry of Higher Education, Research, and the Arts (to TB). GHMS is a recipient of MEXT scholarship.
- Cohen SS (1998) A guide to the polyamines. Oxford University Press, OxfordGoogle Scholar
- Takahashi H, Miller J, Nozaki Y, Sukamto, Takeda M, Shah J, Hase S, Ikegami M, Ehara Y, Dinesh-Kumar SP (2002) RCY1, an Arabidopsis thaliana RPP8/HRT family resistance gene, conferring resistance to cucumber mosaic virus requires salicylic acid, ethylene and a novel signal transduction mechanism. Plant J 32:655–667PubMedCrossRefGoogle Scholar
- Uehara Y, Takahashi Y, Berberich T, Miyazaki A, Takahashi H, Matsui K, Ohme-Takagi M, Saitoh H, Terauchi R, Kusano T (2005) Tobacco ZFT1, a transcriptional repressor with a Cys2/His2 type zinc finger motif that functions in spermine-signaling pathway. Plant Mol Biol 59:435–448PubMedCrossRefGoogle Scholar