Plant Cell Reports

, Volume 31, Issue 5, pp 813–825

Identification and characterization of annexin gene family in rice

  • Sravan Kumar Jami
  • Greg B. Clark
  • Belay T. Ayele
  • Stanley J. Roux
  • P. B. Kirti
Original Paper

Abstract

Plant annexins are Ca2+-dependent phospholipid-binding proteins and are encoded by multigene families. They are implicated in the regulation of plant development as well as protection from drought and other stresses. They are well characterized in Arabidopsis, however no such characterization of rice annexin gene family has been reported thus far. With the availability of the rice genome sequence information, we have identified ten members of the rice annexin gene family. At the protein level, they share 16–64% identity with predicted molecular masses ranging from 32 to 40 kDa. Phylogenetic analysis of rice annexins together with annexins from other monocots led to their classification into five different orthologous groups and share similar motif patterns in their protein sequences. Expression analysis by real-time RT-PCR revealed differential temporal and spatial regulation of these genes. The rice annexin genes are also found to be regulated in seedling stage by various abiotic stressors including salinity, drought, heat and cold. Additionally, in silico analysis of the putative upstream sequences was analyzed for the presence of stress-responsive cis-elements. These results provide a basis for further functional characterization of specific rice annexin genes at the tissue/developmental level and in response to abiotic stresses.

Keywords

Abiotic stress Annexin gene family Phylogeny Rice Seed development 

Supplementary material

299_2011_1201_MOESM1_ESM.doc (40 kb)
Supplementary material 1 (DOC 39 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Sravan Kumar Jami
    • 1
  • Greg B. Clark
    • 2
  • Belay T. Ayele
    • 1
  • Stanley J. Roux
    • 2
  • P. B. Kirti
    • 3
  1. 1.Department of Plant ScienceUniversity of ManitobaWinnipegCanada
  2. 2.Section of Molecular Cell and Developmental BiologyUniversity of TexasAustinUSA
  3. 3.Department of Plant Sciences, School of Life SciencesUniversity of HyderabadHyderabadIndia

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