Plant Cell Reports

, Volume 31, Issue 2, pp 339–347 | Cite as

The wheat gene TaST can increase the salt tolerance of transgenic Arabidopsis

Original Paper


On the basis of the results of gene chip analysis of the salt-tolerant wheat mutant RH8706-49 under conditions of salt stress, we identified and cloned an unknown salt-induced gene TaST (Triticum aestivum salt-tolerant). Real-time quantitative PCR analysis showed that the expression of the gene was induced by salt stress. Transgenic Arabidopsis plants overexpressing the TaST gene showed higher salt tolerance than the wild-type controls. Subcellular localization studies revealed that the protein encoded by this gene was in the nucleus. In comparison with wild-type controls, transgenic Arabidopsis plants accumulated more Ca2+, soluble sugar, and proline and less Na+ under salt stress. Real-time quantitative PCR analysis showed that Arabidopsis plants overexpressing TaST also showed increased expression of many stress-related genes. All these findings indicated that TaST can enhance the salt tolerance of transgenic Arabidopsis plants.


Salt tolerance TaST Wheat Ion content Overexpression 



Triticum aestivum salt-tolerant



This work was supported by the National Natural Science Fund [30971766]; the Hebei Provincial Natural Science Fund [No. C2009000278].

Supplementary material

299_2011_1169_MOESM1_ESM.doc (54 kb)
Supplementary material 1 (DOC 53 kb)


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Xi Huang
    • 1
  • Gang Wang
    • 1
  • Yinzhu Shen
    • 1
  • Zhanjing Huang
    • 1
  1. 1.College of Life Science of Hebei Normal UniversityShijiazhuangPeople’s Republic of China

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