Plant Cell Reports

, Volume 31, Issue 2, pp 339–347 | Cite as

The wheat gene TaST can increase the salt tolerance of transgenic Arabidopsis

Original Paper

Abstract

On the basis of the results of gene chip analysis of the salt-tolerant wheat mutant RH8706-49 under conditions of salt stress, we identified and cloned an unknown salt-induced gene TaST (Triticum aestivum salt-tolerant). Real-time quantitative PCR analysis showed that the expression of the gene was induced by salt stress. Transgenic Arabidopsis plants overexpressing the TaST gene showed higher salt tolerance than the wild-type controls. Subcellular localization studies revealed that the protein encoded by this gene was in the nucleus. In comparison with wild-type controls, transgenic Arabidopsis plants accumulated more Ca2+, soluble sugar, and proline and less Na+ under salt stress. Real-time quantitative PCR analysis showed that Arabidopsis plants overexpressing TaST also showed increased expression of many stress-related genes. All these findings indicated that TaST can enhance the salt tolerance of transgenic Arabidopsis plants.

Keywords

Salt tolerance TaST Wheat Ion content Overexpression 

Abbreviation

TaST

Triticum aestivum salt-tolerant

Notes

Acknowledgments

This work was supported by the National Natural Science Fund [30971766]; the Hebei Provincial Natural Science Fund [No. C2009000278].

Supplementary material

299_2011_1169_MOESM1_ESM.doc (54 kb)
Supplementary material 1 (DOC 53 kb)

References

  1. Armengaud P, Thiery L, Buhot N, Grenier-De MG, Savoure A (2004) Transcriptional regulation of proline biosynthesis in Medicago truncatula reveals developmental and environmental specific features. Physiol Plant 120:442–450PubMedCrossRefGoogle Scholar
  2. Arnon DI (1949) Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15PubMedCrossRefGoogle Scholar
  3. Chinnusamy V, Zhu J (2006) Salt stress signaling and mechanisms of plant salt tolerance. Genet Eng 27:141–177CrossRefGoogle Scholar
  4. Chyzhykova OA, Palladina TO (2006) The role of amino acids and sugars in supporting of osmotic homeostasis in maize seedlings under salinization conditions and treatment with synthetic growth regulators. Ukr Biokhim Zh 78:124–129PubMedGoogle Scholar
  5. Cuin TA, Betts SA, Chalmandrier R, Shabala S (2008) A root’s ability to retain K+ correlates with salt tolerance in wheat. J Exp Bot 59:2697–2706PubMedCrossRefGoogle Scholar
  6. Delauney AJ, Verma DPS (1990) A soybean gene encoding delta 1-pyrroline-5-carboxylate reductase was isolated by functional complementation in Escherichia coliandis found to be osmoregulated. Mol Gen Genet 221:299–305PubMedCrossRefGoogle Scholar
  7. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356CrossRefGoogle Scholar
  8. Espinosa-Ruiz A, Belles JM, Serrano R, Culianez-Macla FA (1999) Arabidopsis thaliana AtHAL3: a flavoprotein related to salt and osmotic tolerance and plant growth. Plant J. 20(5):529–539PubMedCrossRefGoogle Scholar
  9. Gao Z, He X, Zhao B (2010) Overexpressing a putative aquaporin gene from wheat, TaNIP, enhances salt tolerance in transgenic Arabidopsis. Plant Cell Physiol 51(5):767–775PubMedCrossRefGoogle Scholar
  10. Gaxiola RA, Li J, Undurraga S, Dang LM, Allen GJ, Apler SL, Fink GR (2001) Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump. Proc Natl Acad Sci 98(20):11444–11449PubMedCrossRefGoogle Scholar
  11. Ge RC, Chen GP, Zhao BC (2007) Cloning and functional characterization of a wheat serine/threoninekinase gene (TaSTK) related to salt-resistance. Plant Sci 2007(173):55–60CrossRefGoogle Scholar
  12. Golldack D, Dietz KJ (2001) Salt-induced expression of the vacuolar H+-ATPase in the common ice plant is develop-mentally controlled and tissue specific. Plant Physiol 125:1643–1654PubMedCrossRefGoogle Scholar
  13. Hasegawa M, Bressan R, Pardo J (2000) The dawn of plant salt tolerance genetics. Trends Plant Sci 5(8):317–319PubMedCrossRefGoogle Scholar
  14. Hu CAA, Delauney AJ, Verma DPS (1992) A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants. Proc Natl Acad Sci USA 89:9354–9358PubMedCrossRefGoogle Scholar
  15. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods 25:402–408PubMedCrossRefGoogle Scholar
  16. Sairam RK, Tyagi A (2004) Physiology and molecular biology of salinity stress tolerance in plants. Curr Sci 86:407–421Google Scholar
  17. Shi H, Zhu JK (2002) Regulation of expression of the vacuolar Na+/H+ antiporter gene AtNHX1 by salt stress and abscisic acid. Plant Mol Biol 50(3):543–550PubMedCrossRefGoogle Scholar
  18. Su H, Golldack D, Zhao CS, Bohnert HJ (2002) The expression of HAK-type K+ transporters is regulated in response to salinity stress in common ice plant. Plant Physiol 129:1482–1493PubMedCrossRefGoogle Scholar
  19. Sun J, Chen S, Dai S, Wang R (2009) NaCl-induced alternations of cellular and tissue ion fluxes in roots of salt-resistant and salt-sensitive poplar species. Physiol Plant 149:1141–1153Google Scholar
  20. Zhang JS, Xie C, Li ZY, Chen SY (1999) Expression of the plasma membrane H+-ATPase gene in response to salt stress in a rice salt-tolerant mutant and its original variety. Theor Appl Genet 99:1006–1011CrossRefGoogle Scholar
  21. Zhao Q, Zhao YJ, Zhao BC (2009) Cloning and functional analysis of wheat V-H+-ATPase subunit genes. Plant Mol Biol 69:33–46PubMedCrossRefGoogle Scholar
  22. Zhu JK (2002) Salt and drought stress signal transduction in plants. Ann Rev Plant Biol 2002(53):247–273CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Xi Huang
    • 1
  • Gang Wang
    • 1
  • Yinzhu Shen
    • 1
  • Zhanjing Huang
    • 1
  1. 1.College of Life Science of Hebei Normal UniversityShijiazhuangPeople’s Republic of China

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