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Plant Cell Reports

, Volume 31, Issue 1, pp 111–119 | Cite as

Flavonoid production in transgenic hop (Humulus lupulus L.) altered by PAP1/MYB75 from Arabidopsis thaliana L.

  • A. Gatica-Arias
  • M. A. Farag
  • M. Stanke
  • J. Matoušek
  • L. Wessjohann
  • G. WeberEmail author
Original Paper

Abstract

Hop is an important source of secondary metabolites, such as flavonoids. Some of these are pharmacologically active. Nevertheless, the concentration of some classes as flavonoids in wild-type plants is rather low. To enhance the production in hop, it would be interesting to modify the regulation of genes in the flavonoid biosynthetic pathway. For this purpose, the regulatory factor PAP1/AtMYB75 from Arabidopsis thaliana L. was introduced into hop plants cv. Tettnanger by Agrobacterium-mediated genetic transformation. Twenty kanamycin-resistant transgenic plants were obtained. It was shown that PAP1/AtMYB75 was stably incorporated and expressed in the hop genome. In comparison to the wild-type plants, the color of female flowers and cones of transgenic plants was reddish to pink. Chemical analysis revealed higher levels of anthocyanins, rutin, isoquercitin, kaempferol-glucoside, kaempferol-glucoside-malonate, desmethylxanthohumol, xanthohumol, α-acids and β-acids in transgenic plants compared to wild-type plants.

Keywords

Hop Plant transcriptional factors Genetic transformation Secondary metabolites Flavonol glycosides Anthocyanins 

Abbreviations

CaMV

Cauliflower mosaic virus

ESI

Electrospray ionization

HPLC

High-performance liquid chromatography

IAA

Indole-3-acetic acid

LC–MS

Liquid chromatography–mass spectrometry

LC–ESI

Liquid chromatography–electrospray ionization

MS

Murashige and Skoog medium

MSn

Tandem mass spectrometry

nptII

Neomycin phosphotransferase gene

PAP1

Production of anthocyanin pigment 1

PDA

Photodiode array detection

TDZ

1-Phenyl-3-(1,2,3-thiadiazol-5-yl) urea

WT

Wild type

Notes

Acknowledgments

The excellent technical assistance of Ute Born, Kathatrina Alheit and Carolin Aldinger is gratefully acknowledged, as well as the support of the Ministry of Nutrition and Agriculture of the State of Baden-Württemberg (Stuttgart, Germany), Hop Growers Cooperative (Tettnang, Germany) and the Czech Science Foundation (project GACR 521/08/0740). Dr. Mohamed A. Farag thanks the Alexander von Humboldt Foundation for supporting the research at the Leibniz Institute of Plant Biochemistry. The scholarship from the German Academic Exchange Service (DAAD) and partial support from the University of Costa Rica are gratefully acknowledged by A. Gatica.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • A. Gatica-Arias
    • 1
  • M. A. Farag
    • 3
    • 4
  • M. Stanke
    • 1
  • J. Matoušek
    • 2
  • L. Wessjohann
    • 3
  • G. Weber
    • 1
    Email author
  1. 1.Plant Breeding and Biotechnology, Institute for Plant Breeding, Seed Science and Population GeneticsUniversity of HohenheimStuttgartGermany
  2. 2.BC ASCR, v.v.i. IPMBČeské BudějoviceCzech Republic
  3. 3.Department of Bioorganic ChemistryLeibniz Institute of Plant BiochemistryHalle (Saale)Germany
  4. 4.Pharmacognosy DepartmentCollege of Pharmacy, Cairo UniversityCairoEgypt

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