Plant Cell Reports

, Volume 30, Issue 12, pp 2177–2186 | Cite as

The embryo rescue derived intergeneric hybrid between chrysanthemum and Ajania przewalskii shows enhanced cold tolerance

  • Yanming Deng
  • Sumei Chen
  • Fadi Chen
  • Xi Cheng
  • Fei Zhang
Original Paper


Five intergeneric hybrids between the chrysanthemum cultivar ‘Zhongshanjingui’ (as female) and Ajania przewalskii (as male) were obtained with the help of embryo culture. While ‘Zhongshanjingui’ bears a standard anemone type flower and A. przewalskii a non-anemone type one, the inflorescence type of the hybrids varied. The diameter of the hybrids’ flowers was intermediate between those of the parents. The chromosome number of the hybrids was 2n = 45, of which GISH analysis was able to establish that 27 were inherited from ‘Zhongshanjingui’ and the other 18 from A. przewalskii. A combination of various assays was used to show that the cold tolerance of the hybrids was equivalent to that of the highly tolerant A. przewalskii parent. Enhanced cold tolerance was correlated with an increase in free proline and a decrease in malondialdehyde content.


Ajania Chrysanthemum Wide hybridization Embryo rescue Fluorescent GISH Stress tolerance 



Mid-anemone type flower


Standard anemone type flower


Non-anemone type flower


The number of emerged rhizomes


Genomic in situ hybridization


Electrical conductivity


The relative EC


Semi-lethal low temperature






Survival rate



This work was supported by the Basic Infrastructure Construction Plan of Science and Technology of Jiangsu Province (Grant No. BM2008008), the Shanghai Key Science and Technology for Agriculture Promotion Program (2009 No.3-3), the Fundamental Research Funds for the Central Universities (KYJ200907), Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province (CX(10)114).


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Yanming Deng
    • 1
    • 2
  • Sumei Chen
    • 1
  • Fadi Chen
    • 1
  • Xi Cheng
    • 1
  • Fei Zhang
    • 1
  1. 1.College of HorticultureNanjing Agricultural UniversityNanjingChina
  2. 2.Provincial Key Laboratory of AgrobiologyJiangsu Academy of Agricultural SciencesNanjingChina

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