Plant Cell Reports

, Volume 30, Issue 6, pp 1117–1124 | Cite as

Histochemical and immunohistochemical identification of laticifer cells in callus cultures derived from anthers of Hevea brasiliensis

Original Paper

Abstract

Laticifers are highly specialized cells present in over 20 plant families. They are well defined in planta. In vitro development of laticifers was also observed in some plants, but uncertain in the callus cultures of rubber tree, one of the most economically important latex producing plants. In the present study, we provide evidence that laticifer cells present in the callus cultures of rubber tree by histochemical and immunohistochemical studies. They present in the callus mainly as separate non-elongated form, a novel morphology different from the morphology of laticifer cells in planta, excluding their origin from explants. The occurring frequency of laticifer cells in the callus was genotype-dependent and negatively correlated with the somatic embryogenetic ability, suggesting that the presence of laticifer cells in the callus inhibit somatic embryogenesis in tissue culture of rubber tree. The genotypes PR107, RRIM600, Reyan8-79, and Reyan7-33-97 with lower embryogenetic ability compared to Haiken 2 had more laticifer cells, and laticifer clusters were only observed in these genotypes.

Keywords

Hevea brasiliensis Callus Laticifer Immunohistochemistry Histochemistry Latex 

Abbreviation

2,4-D

2,4-Dichlorophenoxyacetic acid

KT

6-Furfurylaminopurine

NAA

1-Naphthaleneacetic acid

SD

Standard deviation

Supplementary material

299_2011_1019_MOESM1_ESM.doc (128 kb)
Autoinfluorescence of callus tissue of rubber tree. a1 and b1 Hand-made sections under normal light. a2 and b2 The same sections as in a1 and b1 under UV light showing the influorescence (DOC 128 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.MOA Key Laboratory for Tropical Crops Biotechnology, Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikouChina

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