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Plant Cell Reports

, Volume 30, Issue 4, pp 665–674 | Cite as

Effects of 2-aminoindan-2-phosphonic acid treatment on the accumulation of salidroside and four phenylethanoid glycosides in suspension cell culture of Cistanche deserticola

  • Gao Sheng Hu
  • Yeon Jae Hur
  • Jing Ming Jia
  • Jai Heon Lee
  • Young Soo Chung
  • Young Byung Yi
  • Dae Jin Yun
  • Soon Ki Park
  • Doh Hoon Kim
Original Paper

Abstract

2-Aminoindan-2-phosphonic acid (AIP), a specific competitive phenylalanine ammonia lyase (PAL) inhibitor was applied to a suspension cell culture of Cistanche deserticola. The effects of AIP treatment on cell growth, PAL activity, contents and yields of total phenolic compound, salidroside and four phenylethanoid glycosides (PheGs) are investigated. The results demonstrated that, 0.5 and 2.0 μM AIP treatments had similar effects on the measurements investigated in this study. AIP treatment resulted in significant decreases in PAL activity, total phenolic compounds content, and PheGs content. Linear regression analysis showed that PAL activity had a high correlation coefficient with the total phenolic compound content and the four PheGs contents. Total PAL activity-time area under curve (AUC) had a high correlation coefficient with the total phenolic compound yield and the yields of five tested compounds in untreated cell samples. In AIP-treated cells, total PAL activity-time AUC retained a high correlation with the total phenolic compound yield and the yields of three tested compounds, echinacoside, acteoside, and tubuloside A, but not salidroside and cistanoside A. The difference could be caused by the different biosynthetic origins of each of the tested compounds. These results demonstrate the important role of PAL in the biosynthesis of PheGs in the suspension cell culture of C. deserticola.

Keywords

Cistanche deserticola Suspension cell culture Phenylethanoid glycosides 2-Aminoindan-2-phosphonic acid (AIP) PAL activity Biosynthesis Salidroside 

Notes

Acknowledgments

This work was supported by the Dong-A University research fund. We would like to thank Professor Jerry Zon who works at the Institute of Organic Chemistry Biochemistry and Biotechnology, Wrocław University of Technology in Poland for providing us AIP. We also would like to show our appreciation to Dr. Nobuhisa Ezaki at the Yomeishu Seizo Company in Japan for giving us standard compounds cistanoside A, acteoside and tubuloside A for our experiments.

Supplementary material

299_2010_997_MOESM1_ESM.doc (161 kb)
Supplementary material 1 (DOC 161 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Gao Sheng Hu
    • 1
  • Yeon Jae Hur
    • 1
  • Jing Ming Jia
    • 2
    • 3
  • Jai Heon Lee
    • 1
  • Young Soo Chung
    • 1
  • Young Byung Yi
    • 1
  • Dae Jin Yun
    • 4
  • Soon Ki Park
    • 5
  • Doh Hoon Kim
    • 1
    • 3
  1. 1.College of Life Science and Natural ResourcesDong-A UniversityBusanRepublic of Korea
  2. 2.School of Traditional Chinese Materia MedicaShenyang Pharmaceutical UniversityShenyangPeople’s Republic of China
  3. 3.Joint Laboratory of Shenyang Pharmaceutical University and Dong-A UniversityShenyangPeople’s Republic of China
  4. 4.Division of Applied Life ScienceGyeongsang National UniversityJinjuRepublic of Korea
  5. 5.Division of Plant BiosciencesKyungpook National UniversityDaeguRepublic of Korea

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