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Plant Cell Reports

, Volume 30, Issue 4, pp 495–503 | Cite as

CaCl2 improves post-drought recovery potential in Camellia sinensis (L) O. Kuntze

  • Hrishikesh Upadhyaya
  • Sanjib Kumar Panda
  • Biman Kumar Dutta
Original Paper

Abstract

Drought stress affects the growth and productivity of the tea plant. However, the damage caused is not permanent. The present investigation studies the effect of CaCl2 on antioxidative responses of tea during post-drought recovery. Increase in dry mass, proline and phenolic content of leaf with decrease in H2O2 and lipid peroxidation and increased activities of enzymes such as SOD, CAT, POX and GR in response to increased foliar CaCl2 concentration are indications for the recovery of stress-induced oxidative damage and thus improving post-stress recovery potential of Camellia sinensis genotypes.

Keywords

Calcium chloride (CaCl2Camellia sinensis (tea) Post-drought recovery (PDR) 

Notes

Acknowledgments

Financial support for the research from the National Tea Research Foundation (NTRF), Kolkatta, (NTRF: 107/07), is gratefully acknowledged. The authors also thank Mr. I. B Ubadhia, Manager, Rosekhandy Tea Estate for providing tea seedlings throughout the experimental work.

References

  1. Asada K, Takahashi M (1987) Production and Scavenging of active oxygen in photosynthesis. In: Kyle DJ, Osmond CB, Arntzen CJ (eds), Photoinhibition. pp 227–287. Elsevier, Amsterdam; New York, OxfordGoogle Scholar
  2. Bars HD, Weatherly PE (1962) A re-examinations of the relative turgidity technique for estimating water deficit in leaves. Aus J Biol Sci 15:413–428Google Scholar
  3. Basu S, Roychoudhury A, Saha PP, Sengupta DN (2010) Differential antioxidative responses of indica rice cultivars to drought stress. Plant Growth Regul 60:51–59CrossRefGoogle Scholar
  4. Bates LS, Waldern RP, Teare I K (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–208Google Scholar
  5. Bian S, Jiang Y (2009) Reactive oxygen species, antioxidant enzyme activities and gene expression patterns in leaves and roots of Kentucky bluegrass in response to drought stress and recovery. Sci Hort 120(2):264–270CrossRefGoogle Scholar
  6. Bouché N, Yellin A, Snedden WA, Fromm H (2005) Plant specific calmodulin-binding proteins. Ann Rev Plant Biol 56:435–466CrossRefGoogle Scholar
  7. Cabrera C, Artacho R, Gimenez R (2006) J Am Coll Nitr. Beneficial effect of green tea–a review 25(2):79–99Google Scholar
  8. Cousson A (2009) Involvement of phospholipase C-independent calcium-mediated abscisic acid signaling during Arabidopsis response to drought. Biol Plant 53(1):53–62CrossRefGoogle Scholar
  9. Evans NH, McAinsh MR, Hetherington AM, Knight MR (2005) ROS perception in Arabidopsis thaliana: the ozone induced calcium response. Plant J 41:615–626PubMedCrossRefGoogle Scholar
  10. Farooqui AHA, Kumar R, Fatima S, Sharma S (2000) Responses of different genotypes of lemon grass (Cymbopogon flexuousus and C. Pendulus) to water stress. J Plant Biol 27:277–282Google Scholar
  11. Foyer CH, Lopez-Delgado H, Dat JF, Scott IM (1997) Hydrogen peroxide and glutathione-associated mechanisms of acclimatory stress tolerance and signaling. Physiol Plant 100:241–254CrossRefGoogle Scholar
  12. Giannopolitis CN, Ries SK (1997) Superoxide dismutase. I. occurrence in higher plants. Plant Physiol 59:309–314CrossRefGoogle Scholar
  13. Grattan SR, Grieve CM (1999) Salinity-mineral nutrient relations in horticultural crops. Sci Hortic 78(1–4):127–157Google Scholar
  14. Griffith OW (1980) Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Ann Biochem 106:207–211CrossRefGoogle Scholar
  15. Handique AC, Manivel L (1990) Selection criteria for drought tolerance in tea. Assam Rev Tea News 79:18–21Google Scholar
  16. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198PubMedCrossRefGoogle Scholar
  17. Herrig V, Ferrarese MLL, Suzuki LS, Rodrigues JD, Ferrarese-Filho O (2002) Peroxidase and phenylalanine ammonia-lyase activities, phenolic acid contents, and allelochemicals-inhibited root growth of soybean. Biol Res 35:59–66Google Scholar
  18. Jaleel CA, Manivannan P, Sankar B, Kishorekumar A, Gopi R, Somasundaram R, Panneerselvam R (2007) Water deficit stress mitigation by calcium chloride in Catharanthus roseus: effects on oxidative stress, proline metabolism and indole alkaloid accumulation. Colloids Surf B Biointerfaces 60:110–116PubMedCrossRefGoogle Scholar
  19. Jeyaramraya PR, Roy RK, Pius PK, Thomas J (2003) Photoassimilatory and photorespiratory behaviour of certain drought tolerant and susceptible tea genotypes. Photosynthetica 41(4):579–582CrossRefGoogle Scholar
  20. Kar M, Mishra D (1976) Catalase, peroxidase and polyphenol oxidase activities during rice leaf senescence. Plant Physiol 57:315–319PubMedCrossRefGoogle Scholar
  21. Kathju S, Vyas SP, Garg BK, Lahiri AN (1988) Fertility induced improvements in performance and metabolism of wheat under different intensities of water stress. In: Proceedings of the International Congress of Plant Physiology, New Delhi, pp 854–858Google Scholar
  22. Kaya C, Higgs D (2002) Calcium nitrate as a remedy for salt-stressed cucumber plants. J Plant Nutr 25(4):861–871CrossRefGoogle Scholar
  23. Kaya C, Kirnak H, Higgs D (2001) Enhancement of growth and normal growth parameters by foliar application of potassium and phosphorus in tomato cultivars grown at high (NaCl) salinity. J Plant Nutr 24(2):357–367CrossRefGoogle Scholar
  24. Khan MN, Siddiqui MH, Mohammad F, Naeem M, Khan MMA (2010) Calcium chloride and gibberellic acid protect linseed (Linum usitatissimum L.) from NaCl stress by inducing antioxidative defence system and osmoprotectant accumulation. Acta Physiol Plant 32:121–132Google Scholar
  25. Kolupaev YE, Akinina GE, Mokrousov AV (2005) Induction of heat tolerance in wheat coleoptiles by calcium ions and its relation to oxidative stress. Russ J Plant Physiol 52:199–204CrossRefGoogle Scholar
  26. Levitt J (1980) Responses of plants to environmental stress, vol I. Academic Press, LondonGoogle Scholar
  27. Lu S, Su W, Li H, Guo Z (2009) Abscisic acid improves drought tolerance of triploid bermudagrass and involves H2O2- and NO-induced antioxidant enzyme activities. Plant Physiol Biochem 47(2):132–138PubMedCrossRefGoogle Scholar
  28. Luo Y, Zhao X, Zhou R, Zuo X, Zhang J, Li Y (2010) Physiological acclimation of two psammophytes to repeated soil drought and rewatering. Acta Physiol Plant. doi: 10.1007/s11738-010-0519-5
  29. Matkowski A, Wolniak D (2005) Plant phenolic metabolites as the free radical scavenger and mutagenesis inhibitors. BMC Plant Biol 5:S23CrossRefGoogle Scholar
  30. Matysik J, Ali BB, Mohanty P (2002) Molecular mechanism of quenching of reactive oxygen species by proline under water stress in plants. Curr Sci 82:525–532Google Scholar
  31. Nayyar H, Kaushal SK (2002) Alleviation of negative effects of water stress in two contrasting wheat genotypes by calcium and abscisic acid. Biol Plant 45(1):65–70CrossRefGoogle Scholar
  32. Noctor G (2006) Metabolic signalling in defence and stress: the central roles of soluble redox couples. Plant Cell Envt 29:409–425CrossRefGoogle Scholar
  33. Oser BL (1979) Hawks physiological chemistry. Mc Graw Hill, NewYork, pp 702–705Google Scholar
  34. Rentel MC, Knight MR (2004) Oxidative stress-induced calcium signaling in Arabidopsis. Plant Physiol 135:1471–1479PubMedCrossRefGoogle Scholar
  35. Ruiz JM, Rivero RM, Lo′ pez-Cantarero I, Romero L (2003) Role of Ca2+ in the metabolism of phenolic compounds in tobacco leaves (Nicotiana tabacum L.). Plant Growth Reg 41:73–177Google Scholar
  36. Sagisaka S (1976) The occurrence of peroxide in a perennial plant Populas gelrica. Plant Physiol 57:308–309PubMedCrossRefGoogle Scholar
  37. Sanders D, Pelloux J, Brownlee C, Harper JF (2002) Calcium at the crossroads of signaling. Plant Cell 14:S401–S417PubMedGoogle Scholar
  38. Sang S, Yang SC, Chi-Tang Ho (2004) Peroxidase mediated oxidation of catechins. Phytochem Rev 3:229–241CrossRefGoogle Scholar
  39. Shao HB, Song WY, Chu LY (2008) Advances of calcium signals involved in plant anti-drought. C R Biologies 331:587–596PubMedCrossRefGoogle Scholar
  40. Siddiqui MH, Al-Whaibi MB, Basalah MO (2010) Interactive effect of calcium and gibberellin on nickel tolerance in relation to antioxidant systems in Triticum aestivum L. Protoplasma. doi: 10.1007/s00709-010-0197-6
  41. Smith IK, Vierheller TL, Thorne CA (1988) Assay of glutathione reductase in crude tissue homogenates using 5, 5′-dithiobis(2-nitrobenzoic acid). Anal Biochem 175:408–413PubMedCrossRefGoogle Scholar
  42. Srivastava RC, Husain MM, Hasan SK, Mohammad A (2000) Green tea polyphenols and tannic acid act as potent inhibitors of phorbol ester-induced nitric oxide generation in rat hepatocytes independent of their antioxidant properties. Cancer Lett 153:1–5PubMedCrossRefGoogle Scholar
  43. Teixeira AF, de Bastos Andrade A, Ferrarese-Filho O, Lucio Ferrarese ML (2006) Role of calcium on phenolic compounds and enzymes related to lignification in soybean (Glycine max L.) root growth. Plant Growth Reg 49:69–76Google Scholar
  44. Upadhyaya H, Panda SK (2004) Responses of Camellia sinensis to drought and rehydration. Biol Plant 48(4):597–600CrossRefGoogle Scholar
  45. Upadhyaya H, Khan MH, Panda SK (2007) Hydrogen peroxide induces oxidative stress in detached leaves of Oryza sativa. Gen Apll Plant Physiol 33(1–2):83–95Google Scholar
  46. Upadhyaya H, Panda SK, Dutta BK (2008) Variation of physiological and antioxidative responses in tea cultivars subjected to elevated water stress followed by rehydration recovery. Acta Physiol Plant 30:457–468CrossRefGoogle Scholar
  47. Verbruggen N, Hermans C (2008) Proline accumulation in plants: a review. Amino Acids 35:753–759PubMedCrossRefGoogle Scholar
  48. Vomáčka L, Pospíšilová J (2003) Rehydration of sugar beet plants after water stress: effect of cytokinins. Biol Plant 46(1):57–62Google Scholar
  49. Wang CQ, Song H (2009) Calcium protects Trifolium repens L. seedlings against cadmium stress. Plant Cell Rep. doi: 10.1007/s00299-009-0734-y
  50. Wang CQ, Wang BS (2007) Ca2+-Calmodulin is involved in betacyanin accumulation induced by darkness in C3 halophyte Suaeda salsa. J Integr Plant Biol 49:1378–1385CrossRefGoogle Scholar
  51. Wang ZY, Cheng SJ, Zhou ZC, Athar M, Khan WA, Bickers DR, Mukhtar H (1989) Antimutagenic activity of green tea polyphenols. Mutat Res 223:273–285PubMedCrossRefGoogle Scholar
  52. Wang CQ, Zhang YF, Liu T (2005) Activity changes of calmodulin and Ca2+-ATPase during low temperature-induced anthocyanin accumulation in Alternanthera bettzickiana. Physiol Plant 124:260–266CrossRefGoogle Scholar
  53. Xu J, Yin HX, Li X (2009) Protective effects of proline against cadmium toxicity in micropropagated hyperaccumulator, Solanum nigrum L. Plant Cell Rep 28:325–333PubMedCrossRefGoogle Scholar
  54. Yoshiba Y, Kiyosue T, Nakashima K, Yamaguchi SK, Shinozaki K (1997) Regulation of level of proline as an osmolyte in plant under water stress. Plant Cell Physiol 18:1095Google Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Hrishikesh Upadhyaya
    • 1
    • 3
  • Sanjib Kumar Panda
    • 1
  • Biman Kumar Dutta
    • 2
  1. 1.Plant Biochemistry and Molecular Biology Laboratory, School of Life SciencesAssam (Central) UniversitySilcharIndia
  2. 2.Microbial and Agricultural Ecology Laboratory, Department of Ecology and Environmental SciencesAssam (Central) UniversitySilcharIndia
  3. 3.Department of Botany and BiotechnologyKarimganj CollegeKarimganjIndia

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