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Plant Cell Reports

, Volume 30, Issue 3, pp 389–398 | Cite as

Overexpression of SlGMEs leads to ascorbate accumulation with enhanced oxidative stress, cold, and salt tolerance in tomato

  • Chanjuan Zhang
  • Junxia Liu
  • Yuyang Zhang
  • Xiaofeng Cai
  • Pengjuan Gong
  • Junhong Zhang
  • Taotao Wang
  • Hanxia Li
  • Zhibiao Ye
Original Paper

Abstract

GDP-Mannose 3′,5′-epimerase (GME; EC 5.1.3.18) catalyses the conversion of GDP-d-mannose to GDP-l-galactose, an important step in the ascorbic acid (AsA) biosynthesis pathway in higher plants. In this study, two members of the GME gene family were isolated from tomato (Solanum lycopersicum). Both SlGME genes encode 376 amino acids and share a 92% similarity with each other. Semi-quantitative RT-PCR indicated that SlGME1 was constantly expressed in various tissues, whereas SlGME2 was differentially expressed in different tissues. Transient expression of fused SlGME1-GFP (green fluorescent protein) and SlGME2-GFP in onion cells revealed the cytoplasmic localisation of the two proteins. Transgenic plants over-expressing SlGME1 and SlGME2 exhibited a significant increase in total ascorbic acid in leaves and red fruits compared with wild-type plants. They also showed enhanced stress tolerance based on less chlorophyll content loss and membrane-lipid peroxidation under methyl viologen (paraquat) stress, higher survival rate under cold stress, and significantly higher seed germination rate, fresh weight, and root length under salt stress. The present study demonstrates that the overexpression of two members of the GME gene family resulted in increased ascorbate accumulation in tomato and improved tolerance to abiotic stresses.

Keywords

Ascorbic acid GDP-Mannose 3′,5′-epimerase Abiotic stress Solanum lycopersicum 

Abbreviations

AsA

Ascorbic acid

GFP

Green fluorescent protein

GME

GDP-Mannose 3′,5′-epimerase

MDA

Malondialdehyde

MV

Methyl viologen

SOE-PCR

Gene splicing with overlap extension PCR

Notes

Acknowledgments

The authors would like to thank Prof. Wenwu Guo for his critical reviews of the manuscript. This study was supported by grants from the State Major Basic Research Development Program (No. 2011CB100600), High-Tech Research and Development Program (No. 2007AA10Z131) and National Natural Science Foundation of China (Nos. 30800756 and 30921002).

Supplementary material

299_2010_939_MOESM1_ESM.doc (594 kb)
Supplementary material 1 (DOC 594 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Chanjuan Zhang
    • 1
  • Junxia Liu
    • 1
  • Yuyang Zhang
    • 1
  • Xiaofeng Cai
    • 1
  • Pengjuan Gong
    • 1
  • Junhong Zhang
    • 1
  • Taotao Wang
    • 2
  • Hanxia Li
    • 2
  • Zhibiao Ye
    • 1
    • 2
  1. 1.The National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanChina
  2. 2.The Key Laboratory of Horticultural Plant BiologyMinistry of Education, Huazhong Agricultural UniversityWuhanChina

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