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Plant Cell Reports

, Volume 29, Issue 10, pp 1187–1201 | Cite as

Characterization of the multiple resistance traits of somatic hybrids between Solanum cardiophyllum Lindl. and two commercial potato cultivars

  • Ramona ThiemeEmail author
  • Elena Rakosy-Tican
  • Marion Nachtigall
  • Jörg Schubert
  • Thilo Hammann
  • Olga Antonova
  • Tatjana Gavrilenko
  • Udo Heimbach
  • Thomas Thieme
Original Paper

Abstract

Interspecific somatic hybrids between commercial cultivars of potato Solanum tuberosum L. Agave and Delikat and the wild diploid species Solanum cardiophyllum Lindl. (cph) were produced by protoplast electrofusion. The hybrid nature of the regenerated plants was confirmed by flow cytometry, simple sequence repeat (SSR), amplified fragment length polymorphism (AFLP), microsatellite-anchored fragment length polymorphism (MFLP) markers and morphological analysis. Somatic hybrids were assessed for their resistance to Colorado potato beetle (CPB) using a laboratory bioassay, to Potato virus Y (PVY) by mechanical inoculation and field trials, and foliage blight in a greenhouse and by field trials. Twenty-four and 26 somatic hybrids of cph + cv. Agave or cph + cv. Delikat, respectively, showed no symptoms of infection with PVY, of which 3 and 12, respectively, were also resistant to foliage blight. One hybrid of cph + Agave performed best in CPB and PVY resistance tests. Of the somatic hybrids that were evaluated for their morphology and tuber yield in the field for 3 years, four did not differ significantly in tuber yield from the parental and standard cultivars. Progeny of hybrids was obtained by pollinating them with pollen from a cultivar, selfing or cross-pollination. The results confirm that protoplast electrofusion can be used to transfer the CPB, PVY and late blight resistance of cph into somatic hybrids. These resistant somatic hybrids can be used in pre-breeding studies, molecular characterization and for increasing the genetic diversity available for potato breeding by marker-assisted combinatorial introgression into the potato gene pool.

Keywords

Colorado potato beetle resistance Resistance to foliage blight Molecular markers Morphology Resistance to Potato virusProtoplast electrofusion 

Abbreviations

AFLP

Amplified fragment length polymorphism

(∆)(r)AUDPC

(delta)(relative) Area under disease progress curve

CPB

Colorado potato beetle

cph

Solanum cardiophyllum

cv.

Commercial variety

DAS-ELISA

Double antibody sandwich enzyme-linked immunosorbent assay

DNA

Desoxyribonucleic acid

EBA

Excised-bud-assay

EBN

Endosperm balance number

GLKS

The Gross Lüsewitz Potato Collections

IC-RT-PCR

Immunocapture reverse transcription polymerase chain reaction

IHAR

Plant breeding and acclimatization Institute

IPK

Leibniz Institute of Plant Genetics and Crop Plant Research

JKI

Julius Kühn Institute

MFLP

Microsatellite-anchored fragment length polymorphism

MRGR

Mean relative growth rate

Norika

Nordring-Kartoffelzucht-und Vermehrungs-GmbH Groß Lüsewitz

PVY

Potato virus Y

RAC

Station de recherche Agroscope Changins

SASA

The Scottish agricultural science agency

SH(s)

Somatic hybrid(s)

SSR

Simple sequence repeat

Notes

Acknowledgments

The German–Romanian and German-Russian Bilateral Projects are acknowledged for supporting part of this research (E. R-T, O. A, T. G). The authors wish to thank Helga Baumann, Hildegard Dreier, Ulrike Busch, Renate Ionasku, Brigitte Deumlich and Petra Hertling for excellent technical assistance. We gratefully thank T. Dixon for his helpful revision of the manuscript.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Ramona Thieme
    • 1
    Email author
  • Elena Rakosy-Tican
    • 2
  • Marion Nachtigall
    • 1
  • Jörg Schubert
    • 3
  • Thilo Hammann
    • 1
  • Olga Antonova
    • 5
  • Tatjana Gavrilenko
    • 5
  • Udo Heimbach
    • 4
  • Thomas Thieme
    • 6
  1. 1.Julius Kühn Institute, Federal Research Centre for Cultivated PlantsInstitute for Breeding Research on Agricultural CropsQuedlinburgGermany
  2. 2.Babeş-Bolyai UniversityCluj-NapocaRomania
  3. 3.Julius Kühn Institute, Federal Research Centre for Cultivated PlantsInstitute for Biosafety of Genetically Modified PlantsQuedlinburgGermany
  4. 4.Julius Kühn Institute, Federal Research Centre for Cultivated PlantsInstitute for Plant Protection in Field Crops and GrasslandBraunschweigGermany
  5. 5.NI Vavilov Institute of Plant IndustrySt. PetersburgRussia
  6. 6.BTL Bio-Test Lab GmbH SagerheideSagerheideGermany

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