Plant Cell Reports

, Volume 29, Issue 10, pp 1131–1137

SbDREB2A, an A-2 type DREB transcription factor from extreme halophyte Salicornia brachiata confers abiotic stress tolerance in Escherichia coli

  • Kapil Gupta
  • Pradeep K. Agarwal
  • M. K. Reddy
  • Bhavanath Jha
Original Paper

Abstract

Dehydration-responsive element binding (DREB) transcription factor plays a key role in plant stress signal transduction pathway. In this study, SbDREB2A has been isolated from the halophyte Salicornia brachiata. SbDREB2A cDNA is 1,062 bp long, encoding protein of 353 amino acids with an estimated molecular mass of 39.37 kDa and a pI of 4.98. On the basis of multiple sequence alignment and phylogenetic analysis, SbDREB2A is classified in A-2 group of the DREB family. The genomic organization confirms that SbDREB2A is an intronless gene. Purified recombinant SbDREB2A protein showed similar binding to both DREs (dehydration-responsive element), ACCGAC and GCCGAC. The transcript expression of SbDREB2A was induced by NaCl, drought and heat stress. The role of SbDREB2A in abiotic stress was studied in E. coli BL21 (DE3). The recombinant E. coli cells exhibited better growth in basal LB medium as well as in supplemented with NaCl, PEG and mannitol. The enhanced growth in recombinant E. coli could be due to the regulation of stress regulated functional genes by this transcription factor. This system can be applied in biotechnological applications, where growth of E. coli can be enhanced under salt stress for efficient recombinant protein production in a short span of time.

Keywords

DREB transcription factor Halophyte Salicornia brachiata Escherichia coli Abiotic stress 

Supplementary material

299_2010_896_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 21 kb)
299_2010_896_MOESM2_ESM.ppt (205 kb)
Supplementary material 2 (PPT 205 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Kapil Gupta
    • 1
  • Pradeep K. Agarwal
    • 1
  • M. K. Reddy
    • 2
  • Bhavanath Jha
    • 1
  1. 1.Discipline of Marine Biotechnology and EcologyCentral Salt and Marine Chemicals Research Institute (Council of Scientific and Industrial Research)BhavnagarIndia
  2. 2.International Centre for Genetic Engineering and BiotechnologyNew DelhiIndia

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