Self-compatibility in ‘Cristobalina’ sweet cherry is not associated with duplications or modified transcription levels of S-locus genes
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Sweet cherry shows S-RNase-based gametophytic self-incompatibility, which prevents self- and cross-fertilization between genetically related individuals. The specificity of the self-incompatible reaction is determined by two genes located in the S-locus. These encode a pistil-expressed ribonuclease (S-RNase) that inhibits self pollen tube growth, and a pollen-expressed F-box protein (SFB) that may be involved in the cytotoxicity of self-S-RNases. Initial genetic and pollination studies in a self-compatible sweet cherry cultivar, ‘Cristobalina’ (S 3 S 6), showed that self-compatibility was caused by the loss of pollen function of both haplotypes (S 3 and S 6). In this study, we further characterize self-compatibility in this genotype by molecular analysis of the S-locus. DNA blot analyses using S-RNase and SFB probes show no duplications of ‘Cristobalina’ S-locus genes or differences in the restriction patterns when compared with self-incompatible cultivars with the same S-genotype. Furthermore, reverse transcriptase-PCR of S-locus genes and quantitative reverse transcription-PCR of SFBs revealed no differences at the transcription level when compared with a self-incompatible genotype. The results of this study show that no differences at the S-locus can be correlated with self-compatibility, indicating the possible involvement of non-S-locus modifiers in self-incompatibility breakdown in this cultivar.
KeywordsPrunus avium Gametophytic self-incompatibility Pollen part mutant
This work was financed by a JSPS Postdoctoral Fellowship awarded to A.W. and research projects JSPS-CSIC: 2004JP0087, Spanish Ministry of Education AGL2007-60130/AGR and INIA- RTA2006-00118.
- Calabrese F, Fenech L, Raimondo A (1984) Kronio: una cultivar di cilegio molto precoce e utocompatibile. Frutticoltura 46:27–30Google Scholar
- Crane MB, Lawrence JC (1929) Genetical and cytological aspects of incompatibility and sterility in cultivated fruits. J Pomol Hortic Sci 7:276–301Google Scholar
- De Nettancourt D (2001) Incompatibility and incongruity in wild and cultivated plants, 2nd edn. Springer, BerlinGoogle Scholar
- Fernández i Martí A, Hanada T, Alonso JM, Yamane H, Ryutaro T, Socias i Company R (2009) A modifier locus affecting the expression of the S-RNase gene could be the cause of breakdown of self-incompatibility in almond. Sex Plant Reprod. doi: 10.1007/s00497-009-0102-7
- Hanada T, Fukuta K, Yamane H, Esumi T, Tao R, Gradziel TM, Dandekar AM, Marti AFI, Alonso JM (2009) Cloning and characterization of a self-compatible S-f Haplotype in almond [Prunus dulcis (Mill.) DA Webb. syn. P. amygdalus Batsch] to resolve previous confusion in its S-f-RNase sequence. Hortscience 44:609–613Google Scholar
- Herrero J (coord.) (1964) Cartografía de las variedades frutales de hueso y pepita. CSIC Aula Dei, ZaragozaGoogle Scholar
- Hugard J (1978) Origine du pollen et varietes pollinisatrices. Le Fruit Belge 381:11–32Google Scholar
- Tao R, Yamane H, Sugiura A, Murayama H, Sassa H, Mori H (1999) Molecular typing of S-alleles through identification, characterization and cDNA cloning for S-RNases in sweet cherry. J Am Soc Hortic Sci 124:224–233Google Scholar
- Watari A, Hanada T, Yamane H, Esumi T, Tao R, Yaegaki H, Yamaguchi M, Beppu K, Kataoka I (2007) A low transcriptional level of S-e-RNase in the S-e-haplotype confers self-compatibility in Japanese plum. J Am Soc Hortic Sci 132:396–406Google Scholar