Plant Cell Reports

, Volume 29, Issue 7, pp 715–721 | Cite as

Self-compatibility in ‘Cristobalina’ sweet cherry is not associated with duplications or modified transcription levels of S-locus genes

  • A. WünschEmail author
  • R. Tao
  • J. I. Hormaza
Original Paper


Sweet cherry shows S-RNase-based gametophytic self-incompatibility, which prevents self- and cross-fertilization between genetically related individuals. The specificity of the self-incompatible reaction is determined by two genes located in the S-locus. These encode a pistil-expressed ribonuclease (S-RNase) that inhibits self pollen tube growth, and a pollen-expressed F-box protein (SFB) that may be involved in the cytotoxicity of self-S-RNases. Initial genetic and pollination studies in a self-compatible sweet cherry cultivar, ‘Cristobalina’ (S 3 S 6), showed that self-compatibility was caused by the loss of pollen function of both haplotypes (S 3 and S 6). In this study, we further characterize self-compatibility in this genotype by molecular analysis of the S-locus. DNA blot analyses using S-RNase and SFB probes show no duplications of ‘Cristobalina’ S-locus genes or differences in the restriction patterns when compared with self-incompatible cultivars with the same S-genotype. Furthermore, reverse transcriptase-PCR of S-locus genes and quantitative reverse transcription-PCR of SFBs revealed no differences at the transcription level when compared with a self-incompatible genotype. The results of this study show that no differences at the S-locus can be correlated with self-compatibility, indicating the possible involvement of non-S-locus modifiers in self-incompatibility breakdown in this cultivar.


Prunus avium Gametophytic self-incompatibility Pollen part mutant 



This work was financed by a JSPS Postdoctoral Fellowship awarded to A.W. and research projects JSPS-CSIC: 2004JP0087, Spanish Ministry of Education AGL2007-60130/AGR and INIA- RTA2006-00118.


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Centro de Investigación y Tecnología Agroalimentaria de AragónZaragozaSpain
  2. 2.Graduate School of AgricultureKyoto UniversityKyotoJapan
  3. 3.Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Estación Experimental “La Mayora”Universidad de Málaga Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC)MalagaSpain

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