Plant Cell Reports

, Volume 29, Issue 5, pp 449–460

Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar

  • Huiquan Zheng
  • Shanzhi Lin
  • Qian Zhang
  • Yang Lei
  • Lu Hou
  • Zhiyi Zhang
Original Paper

Abstract

The PtDrl02 gene belongs to the TIR-NBS gene family in triploid white poplar (Populus tomentosa × P. bolleana) × P. tomentosa. Its expression pattern displays tissue-specificity, and the transcript level can be induced by wounding, methyl jasmonate (MeJA), and salicylic acid (SA). To understand the regulatory mechanism controlling PtDrl02 gene expression, we functionally characterized the PtDrl02 promoter region. Using the β-glucuronidase as a reporter, we found that the PtDrl02 promoter directed gene expression mainly in the aerial parts of the plants and was confined to the cortex tissues of leaf veins, petioles, stems, and stem piths, showing a typical tissue-specific expression pattern. Deletion analysis revealed two positive regulatory regions (−985 to −669 and −669 to −467) responsible for the basal activity of the PtDrl02 promoter. Impressively, the sequence from –669 to –467 was shown to contain cis-element (s) responding to wounding and MeJA, while the promoter region between –244 and 0 could individually display wounding-responsiveness, and the fragment from –467 to –244 was required for SA- and NaCl-inducible expression of the PtDrl02 promoter. Additionally, it was found that the –985 to –669 sequence was the ABA-responding promoter fragment. These results suggested that the PtDrl02 promoter was modulated by multiple cis-regulatory elements in distinct and complex patterns to regulate PtDrl02 gene expression. Our study also suggested that the PtDrl02 gene 5′ untranslated region, as well as a Populus WRKY transcription factor, PtWRKY1, was involved in the regulation of PtDrl02 promoter activities.

Keywords

Populus TIR-NBS cis-element Untranslated region Transcription factor 

Supplementary material

299_2010_834_MOESM1_ESM.tif (2 mb)
Supplemental Fig. 1 Sequence alignment of the W-box-containing fragments within PtDrl02 promoter. The W-box core sequence (TGAC) is marked with double underlines (TIFF 2070 kb)
299_2010_834_MOESM2_ESM.tif (86 kb)
Supplemental Fig. 2 The identified WRKY domains in PtWRKY1 protein. The NCBI () BLASTP program was employed to identify the conserved WRKY domains (TIFhttp://www.ncbi.nlm.nih.gov/F 85 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Huiquan Zheng
    • 1
  • Shanzhi Lin
    • 1
  • Qian Zhang
    • 1
    • 2
  • Yang Lei
    • 1
  • Lu Hou
    • 1
  • Zhiyi Zhang
    • 1
  1. 1.National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry AdministrationBeijing Forestry UniversityBeijingPeople’s Republic of China
  2. 2.Guangdong Academy of ForestryGuangzhouPeople’s Republic of China

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