Isolation and promoter analysis of anther-specific genes encoding putative arabinogalactan proteins in Malus × domestica
- 291 Downloads
In this study, we searched for anther-specific genes involved in male gametophyte development in apple (Malus × domestica Borkh. cv. Fuji) by differential display-PCR. Three full-length cDNAs were isolated, and the corresponding genomic sequences were determined by genome walking. The identified genes showed intronless 228- to 264-bp open reading frames and shared 82–90% nucleotide sequence. Sequence analysis identified that they encoded a putative arabinogalactan protein (AGP) and were designated MdAGP1, MdAGP2, and MdAGP3, respectively. RT (reverse transcriptase)-PCR revealed that the MdAGP genes were selectively expressed in the stamen. Promoter analysis confirmed that the MdAGP3 promoter was capable of directing anther- or pollen-specific expression of the GUS reporter in tobacco and apple. Furthermore, expression of ribosome-inactivating protein under the control of the MdAGP3 promoter induced complete sporophytic male sterility as we had expected.
KeywordsApple (Malus × domestica) Anther specific gene Arabinogalactan protein Male-sterility induction
Open reading frame
Ribosome inactivating protein
This work was supported by a grant (Code 20070301034011) from BioGreen21 program, Rural Development Administration, Republic of Korea.
- Aldwinckle H (1993) Improvement of New York apple varieties and root stocks by genetic engineering. NY Fruit Q Winter:3Google Scholar
- Brown SK (1995) Genetic improvement of apple: the roles of plant breeding and biotechnology. NY Fruit Q 3:2–5Google Scholar
- Cho H, Kim S, Kim M, Kim B (2001) Production of transgenic male sterile tobacco plants with the cDNA encoding a ribosome inactivating protein in Dianthus sinensis L. Mol Cell 11:326–333Google Scholar
- Hrazdina G (1994) Genetic engineering of “Mclntosh” apple to prevent softening during storage. Nk Fruit Q Winter:9Google Scholar
- Knox P, Linstead PJ, Peart J, Cooper C, Roberts K (1991) Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation. Plant J 1:317–326Google Scholar
- Lalanne E, Honys D, Johnson A, Borner G, Lilley K, Dupree P, Grossniklaus U, Twell D (2004) SETH1 and SETH2, two components of the glycosylphosphatidylinositol anchor biosynthetic pathway, are required for pollen germination and tube growth in Arabidopsis. Plant Cell 16:229–240CrossRefPubMedGoogle Scholar