Plant Cell Reports

, Volume 28, Issue 12, pp 1895–1903

Streptomyces scabiei and its toxin thaxtomin A induce scopoletin biosynthesis in tobacco and Arabidopsis thaliana

  • Sylvain Lerat
  • Amadou H. Babana
  • Mohamed El Oirdi
  • Abdelbassed El Hadrami
  • Fouad Daayf
  • Nathalie Beaudoin
  • Kamal Bouarab
  • Carole Beaulieu
Original Paper

Abstract

Streptomyces scabiei is the predominant causal agent of common scab of potato in North America. The virulence of common scab-causing streptomycetes relies on their capacity to synthesize thaxtomins. In this study, the effects of S. scabiei infection and of thaxtomin A, the main toxin produced by S. scabiei, were tested for the elicitation of plant defense molecules in the model plants tobacco (Nicotiana tabacum) and Arabidopsis thaliana. Tobacco leaves infected with spores of S. scabiei strain EF-35 or infiltrated with purified thaxtomin A produced a blue fluorescent compound that was not detected in leaves infiltrated with spores of a S. scabiei mutant deficient in thaxtomin A biosynthesis. Thin layer chromatography and high performance liquid chromatography identified this fluorescent compound as scopoletin, a plant defense phytoalexin. Arabidopsis seedlings grown in liquid medium also excreted scopoletin as a reaction to S. scabiei and thaxtomin A. The effects of the presence of scopoletin on S. scabiei were also investigated. The phytoalexin scopoletin caused a slight reduction of bacterial growth and a severe decrease of thaxtomin A production. Scopoletin was shown to inhibit thaxtomin A production by repression of a gene involved in the toxin biosynthesis.

Keywords

Common scab Model plants Scopoletin Streptomyces scabiei Thaxtomin A 

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Sylvain Lerat
    • 1
  • Amadou H. Babana
    • 2
  • Mohamed El Oirdi
    • 1
  • Abdelbassed El Hadrami
    • 3
  • Fouad Daayf
    • 3
  • Nathalie Beaudoin
    • 1
  • Kamal Bouarab
    • 1
  • Carole Beaulieu
    • 1
  1. 1.Centre SÈVE, Département de BiologieUniversité de SherbrookeSherbrookeCanada
  2. 2.Département de BiologieUniversité de BamakoBamakoMali
  3. 3.Department of Plant Science, 222 Agriculture BuildingUniversity of ManitobaWinnipegCanada

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