Plant Cell Reports

, Volume 28, Issue 12, pp 1837–1846 | Cite as

Production of tetraploid plants of non apomictic citrus genotypes

  • Pablo Aleza
  • José Juárez
  • Patrick Ollitrault
  • Luis NavarroEmail author
Original Paper


Ploidy manipulation in Citrus is a major issue of current breeding programs aiming to develop triploid seedless mandarins to address consumer demands for seedless fruits. The most effective method to obtain triploid hybrids is to pollinate tetraploid non apomictic cultivars with pollen of diploid varieties. Such non apomictic tetraploid lines are not found in the citrus germplasm and need to be created. In this work we describe a new methodology based on in vitro shoot-tip grafting combined with treatment of the micro-grafted shoot-tip with colchicine and oryzalin to achieve chromosome doubling and a dechimerization procedure assisted by flow cytometry. Stable tetraploid plants of Clemenules, Fina and Marisol clementines and Moncada mandarin have been obtained directly from shoot tip grafting combined with colchicine and oryzalin treatments or after dechimerization of mixoploids plants (2x–4x). These stable tetraploid plants have been used in 4x × 2x hybridizations, to recover over 3,250 triploid hybrids in 3 years.


Tetraploid Mixoploid Colchicine Oryzalin Citrus 



This work is jointly financed by the AGL2008-00596-MCI and Prometeo 2008/121 Generalitat Valenciana projects.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Pablo Aleza
    • 1
  • José Juárez
    • 1
  • Patrick Ollitrault
    • 2
  • Luis Navarro
    • 1
    Email author
  1. 1.Centro de Protección Vegetal y BiotecnologíaInstituto Valenciano de Investigaciones Agrarias (IVIA)MoncadaSpain
  2. 2.UPR amélioration génétique des espèces à multiplication végétativeCentre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD)MoncadaSpain

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