Plant Cell Reports

, Volume 28, Issue 12, pp 1799–1816 | Cite as

Monitoring gene expression of potato under salinity using cDNA microarrays

  • Sylvain Legay
  • Didier Lamoureux
  • Jean-François Hausman
  • Lucien Hoffmann
  • Danièle Evers
Original Paper


The molecular response to salt exposure was studied in the leaves of a Solanum tuberosum clone using cDNA microarray. Differentially expressed genes were classified according to their known or predicted function and their expression ratio as compared to the control. The major changes upon a 150 mM NaCl exposure in potato leaves occurred in the photosystem apparatus and Calvin cycle: many transcripts coding for proteins belonging to photosystems I and II and chlorophyll synthesis were repressed. On the other hand, we observed the induction of various kinds of transcription factors implicated in osmotic stress response via ABA-dependent or ABA-independent pathways but also in plant defense pathways. This revealed a crosstalk between abiotic and biotic stress responses during salt exposure, which activated several adaptation mechanisms including heat shock proteins, late embryogenesis abundant, dehydrins and PR proteins. Gene expression changes related to carbohydrate and amino acid metabolism were also observed, pointing at putative modifications at the metabolic level.


NaCl Osmotic stress Solanum tuberosum Abiotic stress Transcriptomics 



The authors wish to thank I. Lefèvre, N. Nicot, K. Van Moer and L. Solinhac for technical assistance and Dr T. Bohn for critical reading of the manuscript. They also acknowledge the Ministry of Finance of Luxembourg for financial support and the International Potato Center (Lima, Peru) for the potato clone.

Supplementary material

299_2009_780_MOESM1_ESM.xls (224 kb)
Supplementary material 1 (XLS 224 kb)
299_2009_780_MOESM2_ESM.doc (25 kb)
Supplementary material 2 (DOC 25 kb)
299_2009_780_MOESM3_ESM.xls (18 kb)
Supplementary material 3 (XLS 18 kb)


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Sylvain Legay
    • 1
  • Didier Lamoureux
    • 1
  • Jean-François Hausman
    • 1
  • Lucien Hoffmann
    • 1
  • Danièle Evers
    • 1
  1. 1.Department EVA Environment and AgrobiotechnologiesCentre de Recherche Public-Gabriel LippmannBelvauxLuxembourg

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