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Plant Cell Reports

, 28:1689 | Cite as

Non-additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between C. reticulata and C. limon: the case of pulp carotenoid biosynthesis pathway

  • Jean Baptiste Bassene
  • Yann Froelicher
  • Claudie Dhuique-Mayer
  • Waffa Mouhaya
  • Rosa Mar Ferrer
  • Gema Ancillo
  • Raphael Morillon
  • Luis Navarro
  • Patrick OllitraultEmail author
Original Paper

Abstract

Allopolyploidy is known to induce novel patterns of gene expression and often gives rise to new phenotypes. Here we report on the first attempt to relate phenotypic inheritance in an allotetraploid somatic hybrid with gene expression. Carotenoid compounds in the fruit pulp of the two parental species and the hybrid were evaluated quantitatively by HPLC. Only very low levels of β-carotene and β-cryptoxanthin were observed in Citrus limon, while β-cryptoxanthin was a major component of C. reticulata, which also displayed high levels of phytoene, phytofluene, β-carotene, lutein, zeaxantin and violaxanthin. Total carotenoid content in mandarin juice sacs was 60 times greater than that in lemon. The allotetraploid hybrid produced all the same compounds as mandarin but at very low levels. Transgressive concentration of abscisic acid (ABA) was observed in the somatic hybrid. Real-time RT-PCR of total RNA from juice sacs was used to study expression of seven genes (CitDxs, CitPsy, CitPds, CitZds, CitLcy-b, CitChx-b, and CitZep) of the carotenoid biosynthetic pathway and two genes (CitNced1 and CitNced2) involved in abscisic acid synthesis from carotenoid. Gene expression was significantly higher for mandarin than lemon for seven of the nine genes analyzed. Lemon under expression was partially dominant in the somatic hybrid for three upstream steps of the biosynthetic pathway, particularly for CitDxs. Transgressive over expression was observed for the two CitNced genes. A limitation of the upstream steps of the pathway and a downstream higher consumption of carotenoids may explain the phenotype of the somatic hybrid.

Keywords

Citrus Somatic hybrid Carotenoid Gene expression Inheritance 

Notes

Acknowledgments

This study was subsided by the Collectivité Territoriale de Corse, the ‘Triploid mandarin breeding project (Proyectos de Investigación Fundamental no orientada; AGL 2008-00596) and the ‘Biotechnologia de citricos’ project (Prometeo 2008/12).

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Jean Baptiste Bassene
    • 1
  • Yann Froelicher
    • 1
  • Claudie Dhuique-Mayer
    • 2
  • Waffa Mouhaya
    • 3
  • Rosa Mar Ferrer
    • 4
  • Gema Ancillo
    • 4
  • Raphael Morillon
    • 3
  • Luis Navarro
    • 4
  • Patrick Ollitrault
    • 3
    • 3
    Email author
  1. 1.UPR Amélioration génétique des espèces à multiplication végétativeCIRAD (Centre de Coopération Internationale en Recherche Agronomique pour le Développement)San GiulianoFrance
  2. 2.UMR QUALISUDCIRAD (Centre de Coope´ration Internationale en Recherche Agronomique pour le Développement)Montpellier Cedex 5France
  3. 3.UPR Amélioration génétique des espèces à multiplication végétativeCIRAD (Centre de Coopération Internationale en Recherche Agronomique pour le Développement), IVIAMoncadaSpain
  4. 4.Centro de Proteccion vegetal y biotechnologiaInstituto Valenciano de Investigaciones Agrarias (IVIA)MoncadaSpain

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