Plant Cell Reports

, Volume 28, Issue 8, pp 1279–1287 | Cite as

Stress-related variation in antioxidative enzymes activity and cell metabolism efficiency associated with embryogenesis induction in isolated microspore culture of triticale (x Triticosecale Wittm.)

  • Iwona ŻurEmail author
  • Ewa Dubas
  • Elżbieta Golemiec
  • Magdalena Szechyńska-Hebda
  • Gabriela Gołębiowska
  • Maria Wędzony
Original Paper


Isolated microspore cultures of two spring triticale (x Triticosecale Wittm.) cultivars were used to examine the effect of various stress treatments (either high—32°C or low—5°C temperature with or without nitrogen/carbohydrate starvation) applied to excised anthers on the effectiveness of microspore embryogenesis induction. To quantify the effects of pretreatment conditions, the activity of antioxidative enzymes (catalase, peroxidase and superoxide dismutase) together with respiration rate and heat emission were measured. It was observed that heat shock treatment applied as the only one stress factor increased the activity of antioxidative enzymes which suggests intensive generation of reactive oxygen species. Such pretreatment effectively triggered microspore reprogramming but drastically decreased microspore viability. After low temperature treatment, the activity of antioxidative enzymes was similar to the control subjected only with the stress originated from the transfer to in vitro culture conditions. This pretreatment decreased the number of microspores entering embryogenesis but sustained cell viability and this effect prevailed in the final estimation of microspore embryogenesis effectiveness. For both, low- and high-temperature treatments, interaction with starvation stress was beneficial increasing microspore viability (at 5°C) or efficiency of embryogenesis induction (at 32°C). The latter treatment significantly reduced cell metabolic activity. Physiological background of these effects seems to be different and some hypothetical explanations have been discussed. Received data indicate that in triticale, anther preculture conditions could generate oxidative stress and change the cell metabolic activity which could next be reflected in the cell viability and the efficiency of microspore embryogenesis.


Antioxidative enzymes Metabolic activity Oxidative stress Microspore embryogenesis 



Abscisic acid




Doubled haploids


Dry weight


Ethylenediaminetetraacetic acid


Embryo-like structures


Hydrogen peroxide


Potassium phosphate buffer


α-Naphthaleneacetic acid


Photosynthetic active radiation




Reactive oxygen species


Superoxide dismutase



The research was supported by the project KBN23/E189/SPB/COST/P06/Dz585/2002-2005.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Iwona Żur
    • 1
    Email author
  • Ewa Dubas
    • 1
  • Elżbieta Golemiec
    • 1
  • Magdalena Szechyńska-Hebda
    • 1
  • Gabriela Gołębiowska
    • 1
    • 2
  • Maria Wędzony
    • 1
    • 2
  1. 1.Institute of Plant PhysiologyPolish Academy of SciencesKrakówPoland
  2. 2.Pedagogical University of KrakówKrakówPoland

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