Plant Cell Reports

, Volume 28, Issue 8, pp 1169–1177 | Cite as

Callus, shoot and hairy root formation in vitro as affected by the sensitivity to auxin and ethylene in tomato mutants

  • Joni Esrom Lima
  • Vagner Augusto Benedito
  • Antonio Figueira
  • Lázaro Eustáquio Pereira Peres
Original Paper

Abstract

We analyzed the impact of ethylene and auxin disturbances on callus, shoots and Agrobacterium rhizogenes-induced hairy root formation in tomato (Solanum lycopersicum L.). The auxin low-sensitivity dgt mutation showed little hairy root initiation, whereas the ethylene low-sensitivity Nr mutation did not differ from the control Micro-Tom cultivar. Micro-Tom and dgt hairy roots containing auxin sensitivity/biosynthesis rol and aux genes formed prominent callus onto media supplemented with cytokinin. Under the same conditions, Nr hairy roots did not form callus. Double mutants combining Rg1, a mutation conferring elevated shoot formation capacity, with either dgt or Nr produced explants that formed shoots with little callus proliferation. The presence of rol + aux genes in Rg1 hairy roots prevented shoot formation. Taken together, the results suggest that although ethylene does not affect hairy root induction, as auxin does, it may be necessary for auxin-induced callus formation in tomato. Moreover, excess auxin prevents shoot formation in Rg1.

Keywords

Agrobacterium Lycopersicon Regeneration Rg1 rol genes Solanum 

Abbreviations

MT

Micro-Tom

Rg1

Regeneration locus 1

dgt

diageotropica

Nr

Never ripe

rol

root loci

pRi

Root inductor plasmid

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Joni Esrom Lima
    • 2
    • 3
  • Vagner Augusto Benedito
    • 2
    • 4
  • Antonio Figueira
    • 2
  • Lázaro Eustáquio Pereira Peres
    • 1
  1. 1.Department of Biological Sciences (LCB), Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ)Universidade de São Paulo (USP)PiracicabaBrazil
  2. 2.Centro de Energia Nuclear na Agricultura (CENA)USPPiracicabaBrazil
  3. 3.Institut für PflanzenernährungUniversität HohenheimStuttgartGermany
  4. 4.Plant Biology DivisionSamuel Roberts Noble FoundationArdmoreUSA

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